Publication:
Can local enteric neural stimulation evoke 5-HT release from EC cells?
Can local enteric neural stimulation evoke 5-HT release from EC cells?
dc.contributor.author | Bertrand, P. P | en_US |
dc.date.accessioned | 2021-11-25T13:34:28Z | |
dc.date.available | 2021-11-25T13:34:28Z | |
dc.date.issued | 2007 | en_US |
dc.description.abstract | Background/Aims: Enterochromaffin (EC) cells are sensors in the intestinal lumen that detect chemical or mechanical stimuli and respond with the release of serotonin (5-HT). 5-HT can activate local reflexes but whether local activation of enteric neural circuits also evoke 5-HT release is unclear. Methods: Recordings were made from full-thickness preparations of guinea pig ileum using electrochemical techniques with carbon fibre electrodes placed in the mucosa to measure local concentrations of 5-HT. The tension in the circular muscle (CM) was recorded with a force transducer. Amplitude and time course of local 5-HT release events and muscle contractions were measured and compared using paired Student’s t-test with Bonferroni’s correction for multiple comparisons. Results: Stretch of the CM caused 5-HT release and reflex contraction of the CM. Focal electrical stimulation of the intestine near to the carbon fibre electrode evoked 5-HT release and caused a local contraction in the ring of CM where the recording site was located. Paralysis of the smooth muscle with papaverine (100 mm; n55), sodium nitroprusside (100 mm; n55) or isoproterenol (1 mm; n55) significantly reduced (o25% of control) the stretch-evoked release of 5-HT. Similarly, isoproterenol (1 mm; n54) or sodium nitroprusside (100 mm; n53), abolished (o10% of control) the electrically evoked release of 5-HT. Atropine (1 mm; n53), which would be expected to block muscarinic input to the EC cell, did not reduce stretch-evoked 5-HT release. Conclusion: The present study provides direct evidence that activated enteric nerves are not responsible for the 5-HT release seen during local reflexes. There was little residual 5-HT release in response to stretch or electrical stimulation of the nerves in paralysed preparations and atropine did not reduce reflex-evoked 5-HT release. Together, these data suggest that mechnical, and not neural, stimuli provide an important excitatory input to the EC cells. | en_US |
dc.identifier.issn | 1350-1925 | en_US |
dc.identifier.uri | http://hdl.handle.net/1959.4/40003 | |
dc.language | English | |
dc.language.iso | EN | en_US |
dc.publisher | Blackwell Publishing | en_US |
dc.rights | CC BY-NC-ND 3.0 | en_US |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/3.0/au/ | en_US |
dc.source | Legacy MARC | en_US |
dc.subject.other | serotonin (5-HT) | en_US |
dc.subject.other | enteric reflexes | en_US |
dc.subject.other | sensory transduction | en_US |
dc.subject.other | enterochromaffin cells | en_US |
dc.title | Can local enteric neural stimulation evoke 5-HT release from EC cells? | en_US |
dc.type | Conference Paper | en |
dcterms.accessRights | metadata only access | |
dspace.entity.type | Publication | en_US |
unsw.accessRights.uri | http://purl.org/coar/access_right/c_14cb | |
unsw.identifier.doiPublisher | http://dx.doi.org/10.1111/j.1350-1925.2007.01011.x | en_US |
unsw.publisher.place | Oxford | en_US |
unsw.relation.faculty | Medicine & Health | |
unsw.relation.ispartofconferenceLocation | Jeju Island, Korea | en_US |
unsw.relation.ispartofconferenceName | 21st International Symposium on Neurogastroentrology and Motility | en_US |
unsw.relation.ispartofconferenceProceedingsTitle | Neurogastroenterology & Motility, Vol 19 | en_US |
unsw.relation.ispartofconferenceYear | 2007 | en_US |
unsw.relation.ispartofpagefrompageto | 20-21 | en_US |
unsw.relation.originalPublicationAffiliation | Bertrand, P. P, Medical Sciences, Faculty of Medicine, UNSW | en_US |
unsw.relation.school | School of Medical Sciences | * |