Elucidation of the underlying mechanisms governing algae and cyanobacteria separation using the PosiDAF process

Abstract

The novel PosiDAF process that uses cationic-polymer modified bubbles has been suggested as an alternative to conventional dissolved air flotation for the separation of algae. However, a cationic PosiDAF effluent compared to an anionic influent as detected by charge measurements indicated that effluent contained high polymer residuals and was undesirable. To prevent this, prior research investigated stronger polymer-bubble adhesion by developing hydrophobically modified polymers (HMPs) of poly(dimethylaminoethyl methacrylate) (PDMAEMA). However, while bench scale tests using the HMPs were successful, commercially available poly(diallyldimethylammonium chloride) (PDADMAC) outperformed the HMPs in pilot scale, suggesting that PDADMAC has a more suitable polymer backbone. Moreover, algal organic matter (AOM) released by cells, particularly biopolymers, was observed to influence cell separation. Further research is required to investigate alternate polymers and to determine more precisely the underlying mechanisms governing polymer-bubble-AOM interactions in PosiDAF. In this study, PDADMAC was modified with various aromatic and aliphatic pendant groups to generate several HMPs. Select HMPs of PDADMAC and previously investigated PDMAEMA were compared to evaluate polymer-bubble attachment and PosiDAF performance to separate algae and cyanobacteria. The composition of AOM, particularly biopolymers from each strain tested was characterised and their influence examined by conducting experiments with various AOM, protein and carbohydrate concentrations. The results showed that HMP coated bubbles had lower surface tensions and consequently, anionic effluents and strong polymer-bubble adhesion. Concurrently, cell separation was either comparable, or slightly better between HMPs. However, separation effectiveness varied for several algae, indicating that AOM impacted separation. Moreover, cell separation of the strains increased to > 95% when exudates from the best separated strain were added. On bulk and molecular characterisation of the cultures, the best separated strain was found to be biopolymer rich in comparison to the other strains. Hence, proteins and carbohydrates were dosed to study their influence and were observed to either depress or enhance the flotation depending on their character. It was concluded that the interplay of biopolymers with polymer-bubble-cell was responsible for the variations in cell removal observed across several strains. Overall, with a well-defined AOM character and low polymer residual in effluent, PosiDAF has been demonstrated as a robust and sustainable process.