Identification of mammalian lipid material by high performance liquid chromatography and Fourier transform ion cyclotron resonance mass spectrometry

Abstract

The analysis of the exact compositions of human lipids is of great importance and can lead to a better understanding of the role of lipids in heart diseases and diabetes. Reverse phase and normal phase high performance liquid chromatography has been used for the analysis of lipid material and applied to beef dripping. Electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry has been used to investigate the free fatty acids, monoacylglycerides, diacylglycerides and triacylglycerides present in lard and beef dripping with the general conclusion that this is a semi quantitative method. Collision induced dissociation of di- and triacylglycerides was performed using Fourier transform ion cyclotron resonance mass spectrometry with the energy of activation for the fragmentation process investigated and compared using density functional theory and Rice Ramsperger Kassel Marcus statistical theory of fragmentation. The application of tandem mass spectrometry to mice and human cell cultures and plaques from the abdominal aorta of rabbits was conducted using normal phase high performance liquid chromatography with electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry and quadrupole time of flight mass spectrometry.