The Effects of Bile on Campylobacter concisus Growth and Protein Expression

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Copyright: Sapwell, Nicholas
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Abstract
Campylobacter concisus has been considered as a possible triggering agent of Inflammatory Bowel Disease (IBD). This aim of this project was to determine the impact of bile on the growth and survival of C. concisus strains isolated from patients with IBD and controls. Furthermore, the effects of bile on C. concisus protein secretion were examined and cloning of a C. concisus gene that is related to bile exposure was attempted. C. concisus strains (42 strains) previously isolated from saliva samples of patients with IBD and healthy individuals were examined. C. concisus strains were cultured on horse blood agar plates containing 2% ox bile (HBA-B). The inhibition of bile on C. concisus growth was further quantified by the examination of the colony forming unit (CFU). C. concisus protein secretion in response to bile exposure was examined using mass spectrometry analysis. Gene cloning was performed following standard procedures. Of the 42 C. concisus strains examined, 45.2% strains (19/42) were resistant, 38.1% strains (16/42) were tolerant and 16.7% (7/42) were sensitive to 2% bile. The rates of resistant, tolerant and sensitive to bile of C. concisus strains isolated from patients with CD, UC and healthy controls were not statistically significant (P> 0.05). The degree of concentration dependent bile sensitivity varies between strains of C. concisus strains. The presence of 2% bile greatly inhibited the growth of C. concisus strains; the percentage CFU of bile resistant strains on HBA-B and HBA plates were 0.01% - 6.34%. Furthermore, bile impacted C. concisus growth in a time and concentration dependent manner.
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Sapwell, Nicholas
Supervisor(s)
Zhang, Li
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Publication Year
2014
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Thesis
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Masters Thesis
UNSW Faculty
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