A modular click approach to glycosylated polymeric beads: Design, synthesis and preliminary lectin, recognition studies Chen, Gaojian en_US Tao, L. en_US Mantovani, G. en_US Geng, J. en_US Nystrom, D. en_US Haddleton, D. M. en_US 2021-11-25T14:08:49Z 2021-11-25T14:08:49Z 2007 en_US
dc.description.abstract Covalent immobilization of a range of carbohydrate derivatives onto polymeric resin beads is described. Copper-catalyzed Huisgen [2 + 3] cycloaddition (often termed click chemistry) was used to graft mannose-containing azides to complementarily functionalized alkyne surfaces, namely (a) Wang resin or (b) Rasta particles consisting of a clickable alkyne polymer loose outer shell and a Wang resin inner core. For the second approach, Wang resin beads were first converted into immobilized living radical polymerization initiators with subsequent polymerization of trimethylsilanyl-protected propargyl methacrylate followed by deprotection with TBAF to yield the desired polyalkyne clickable scaffold. The appropriate (x-mannopyranoside azide was then clicked onto the bead to give a mannose functionalized Rasta resin. IR, gel-phase H-1 NMR, and elemental analysis have been used to characterize the modified resins. The binding abilities of these D-mannose-modified particles were subsequently tested using fluorescein-labeled Concanavalin A (Con A), a lectin that binds certain mannose-containing molecules. Preliminary results indicated that the novel glyco-hybrid materials presented in this work are able to efficiently recognize mannose-binding model lectins such as Con A, opening the way for their potential application in affinity chromatography, sensors, and other protein recognition/separation fields. en_US
dc.language English
dc.language.iso EN en_US
dc.rights CC BY-NC-ND 3.0 en_US
dc.rights.uri en_US
dc.source Legacy MARC en_US
dc.subject.other Polymeric resin beads. en_US
dc.subject.other Modular click. en_US
dc.subject.other Glycosylated polymeric beads. en_US
dc.subject.other Chemical science. en_US
dc.subject.other Engineering. en_US
dc.title A modular click approach to glycosylated polymeric beads: Design, synthesis and preliminary lectin, recognition studies en_US
dc.type Journal Article en
dcterms.accessRights metadata only access
dspace.entity.type Publication en_US
unsw.identifier.doiPublisher en_US
unsw.relation.faculty Engineering
unsw.relation.ispartofissue 21 en_US
unsw.relation.ispartofjournal MACROMOLECULES en_US
unsw.relation.ispartofpagefrompageto 7513-7520 en_US
unsw.relation.ispartofvolume 40 en_US
unsw.relation.originalPublicationAffiliation Chen, Gaojian, Chemical Sciences & Engineering, Faculty of Engineering, UNSW en_US
unsw.relation.originalPublicationAffiliation Tao, L. en_US
unsw.relation.originalPublicationAffiliation Mantovani, G. en_US
unsw.relation.originalPublicationAffiliation Geng, J. en_US
unsw.relation.originalPublicationAffiliation Nystrom, D. en_US
unsw.relation.originalPublicationAffiliation Haddleton, D. M. en_US School of Chemical Engineering *
Resource type