Publication:
A modular click approach to glycosylated polymeric beads: Design, synthesis and preliminary lectin, recognition studies
A modular click approach to glycosylated polymeric beads: Design, synthesis and preliminary lectin, recognition studies
dc.contributor.author | Chen, Gaojian | en_US |
dc.contributor.author | Tao, L. | en_US |
dc.contributor.author | Mantovani, G. | en_US |
dc.contributor.author | Geng, J. | en_US |
dc.contributor.author | Nystrom, D. | en_US |
dc.contributor.author | Haddleton, D. M. | en_US |
dc.date.accessioned | 2021-11-25T14:08:49Z | |
dc.date.available | 2021-11-25T14:08:49Z | |
dc.date.issued | 2007 | en_US |
dc.description.abstract | Covalent immobilization of a range of carbohydrate derivatives onto polymeric resin beads is described. Copper-catalyzed Huisgen [2 + 3] cycloaddition (often termed click chemistry) was used to graft mannose-containing azides to complementarily functionalized alkyne surfaces, namely (a) Wang resin or (b) Rasta particles consisting of a clickable alkyne polymer loose outer shell and a Wang resin inner core. For the second approach, Wang resin beads were first converted into immobilized living radical polymerization initiators with subsequent polymerization of trimethylsilanyl-protected propargyl methacrylate followed by deprotection with TBAF to yield the desired polyalkyne clickable scaffold. The appropriate (x-mannopyranoside azide was then clicked onto the bead to give a mannose functionalized Rasta resin. IR, gel-phase H-1 NMR, and elemental analysis have been used to characterize the modified resins. The binding abilities of these D-mannose-modified particles were subsequently tested using fluorescein-labeled Concanavalin A (Con A), a lectin that binds certain mannose-containing molecules. Preliminary results indicated that the novel glyco-hybrid materials presented in this work are able to efficiently recognize mannose-binding model lectins such as Con A, opening the way for their potential application in affinity chromatography, sensors, and other protein recognition/separation fields. | en_US |
dc.identifier.uri | http://hdl.handle.net/1959.4/42103 | |
dc.language | English | |
dc.language.iso | EN | en_US |
dc.rights | CC BY-NC-ND 3.0 | en_US |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/3.0/au/ | en_US |
dc.source | Legacy MARC | en_US |
dc.subject.other | Polymeric resin beads. | en_US |
dc.subject.other | Modular click. | en_US |
dc.subject.other | Glycosylated polymeric beads. | en_US |
dc.subject.other | Chemical science. | en_US |
dc.subject.other | Engineering. | en_US |
dc.title | A modular click approach to glycosylated polymeric beads: Design, synthesis and preliminary lectin, recognition studies | en_US |
dc.type | Journal Article | en |
dcterms.accessRights | metadata only access | |
dspace.entity.type | Publication | en_US |
unsw.accessRights.uri | http://purl.org/coar/access_right/c_14cb | |
unsw.identifier.doiPublisher | http://dx.doi.org/10.1021/ma071362v | en_US |
unsw.relation.faculty | Engineering | |
unsw.relation.ispartofissue | 21 | en_US |
unsw.relation.ispartofjournal | MACROMOLECULES | en_US |
unsw.relation.ispartofpagefrompageto | 7513-7520 | en_US |
unsw.relation.ispartofvolume | 40 | en_US |
unsw.relation.originalPublicationAffiliation | Chen, Gaojian, Chemical Sciences & Engineering, Faculty of Engineering, UNSW | en_US |
unsw.relation.originalPublicationAffiliation | Tao, L. | en_US |
unsw.relation.originalPublicationAffiliation | Mantovani, G. | en_US |
unsw.relation.originalPublicationAffiliation | Geng, J. | en_US |
unsw.relation.originalPublicationAffiliation | Nystrom, D. | en_US |
unsw.relation.originalPublicationAffiliation | Haddleton, D. M. | en_US |
unsw.relation.school | School of Chemical Engineering | * |