Publication:
A Sanger/Pyrosequencing Hybrid Approach for Generation of High Quality Draft Assemblies of Marine Microbial Genomes

dc.contributor.author Goldberg, SM en_US
dc.contributor.author Johnson, J en_US
dc.contributor.author Busam, D en_US
dc.contributor.author Feldblyum, T en_US
dc.contributor.author Ferriera, S en_US
dc.contributor.author Friedman, R en_US
dc.contributor.author Halpern, A en_US
dc.contributor.author Khouri, H en_US
dc.contributor.author Kravitz, SA en_US
dc.contributor.author Lauro, FM en_US
dc.contributor.author Li, K en_US
dc.contributor.author Rogers, H-Y en_US
dc.contributor.author Strausberg, R en_US
dc.contributor.author Sutton, G en_US
dc.contributor.author Tallon, L en_US
dc.contributor.author Thomas, Torsten en_US
dc.contributor.author Venter, E en_US
dc.contributor.author Frazier, M en_US
dc.contributor.author Venter, JC en_US
dc.date.accessioned 2021-11-25T13:24:23Z
dc.date.available 2021-11-25T13:24:23Z
dc.date.issued 2006 en_US
dc.description.abstract Since its introduction a decade ago, whole-genome shotgun sequencing (WGS) has been the main approach for producing cost-effective and high-quality genome sequence data. Until now, the Sanger sequencing technology that has served as a platform for WGS has not been truly challenged by emerging technologies. The recent introduction of the pyrosequencing-based 454 sequencing platform (454 Life Sciences, Branford, CT) offers a very promising sequencing technology alternative for incorporation in WGS. In this study, we evaluated the utility and cost-effectiveness of a hybrid sequencing approach using 3730xl Sanger data and 454 data to generate higher-quality lower-cost assemblies of microbial genomes compared to current Sanger sequencing strategies alone. en_US
dc.identifier.uri http://hdl.handle.net/1959.4/39565
dc.language English
dc.language.iso EN en_US
dc.rights CC BY-NC-ND 3.0 en_US
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/3.0/au/ en_US
dc.source Legacy MARC en_US
dc.title A Sanger/Pyrosequencing Hybrid Approach for Generation of High Quality Draft Assemblies of Marine Microbial Genomes en_US
dc.type Journal Article en
dcterms.accessRights metadata only access
dspace.entity.type Publication en_US
unsw.accessRights.uri http://purl.org/coar/access_right/c_14cb
unsw.identifier.doiPublisher http://dx.doi.org/10.1073/pnas.0604351103 en_US
unsw.relation.faculty Science
unsw.relation.ispartofissue 30 en_US
unsw.relation.ispartofjournal Proceedings of the National Academy of Science U S A en_US
unsw.relation.ispartofpagefrompageto 11240-11245 en_US
unsw.relation.ispartofvolume 103 en_US
unsw.relation.originalPublicationAffiliation Goldberg, SM en_US
unsw.relation.originalPublicationAffiliation Johnson, J en_US
unsw.relation.originalPublicationAffiliation Busam, D en_US
unsw.relation.originalPublicationAffiliation Feldblyum, T en_US
unsw.relation.originalPublicationAffiliation Ferriera, S en_US
unsw.relation.originalPublicationAffiliation Friedman, R en_US
unsw.relation.originalPublicationAffiliation Halpern, A en_US
unsw.relation.originalPublicationAffiliation Khouri, H en_US
unsw.relation.originalPublicationAffiliation Kravitz, SA en_US
unsw.relation.originalPublicationAffiliation Lauro, FM en_US
unsw.relation.originalPublicationAffiliation Li, K en_US
unsw.relation.originalPublicationAffiliation Rogers, H-Y en_US
unsw.relation.originalPublicationAffiliation Strausberg, R en_US
unsw.relation.originalPublicationAffiliation Sutton, G en_US
unsw.relation.originalPublicationAffiliation Tallon, L en_US
unsw.relation.originalPublicationAffiliation Thomas, Torsten, Biotechnology & Biomolecular Sciences, Faculty of Science, UNSW en_US
unsw.relation.originalPublicationAffiliation Venter, E en_US
unsw.relation.originalPublicationAffiliation Frazier, M en_US
unsw.relation.originalPublicationAffiliation Venter, JC en_US
unsw.relation.school School of Biotechnology & Biomolecular Sciences *
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