Nitric oxide synthase isoforms during fracture healing Zhu, Wei en_US Diwan, Ashish en_US Lin, J en_US Murrell, George en_US 2021-11-25T15:30:13Z 2021-11-25T15:30:13Z 2001 en_US
dc.description.abstract We have shown previously that nitric oxide (NO) has regulatory effects on fracture healing. Our aim here was to investigate the temporal expression patterns of the three NO synthase (NOS) isoforms that are responsible for the generation of NO by semiquantitative competitive polymerase chain reaction (PCR) and immunoblot analysis after femoral fractures in rats. We found that 4 days after fracture, there were increases in the levels of messenger RNA (mRNA) for all three NOS isoforms, with peaks for the inducible NOS (iNOS; 35-fold increase, p < 0.05) at day 4, the endothelial NOS (eNOS; 5-fold increase, p < 0.05) at day 7, and the neuronal NOS (bNOS; 16-fold increase, p < 0.05) at day 21. At a protein level, the time course expression of NOS isoforms was consistent with the results of those at the mRNA level. In addition, we have previously reported a 2.5-fold increase in NOS activity detected by [3H]arginine to [3H]citrulline conversion at day 15 compared with that at day 4 after fracture. The findings that the expression of NOS isoforms during fracture healing is type specific and time dependent are important and may have clinical applications in the regulation of bone repair by NOS inhibitors or stimulators at different stages after injury. en_US
dc.identifier.issn 0884-0431 en_US
dc.language English
dc.language.iso EN en_US
dc.rights CC BY-NC-ND 3.0 en_US
dc.rights.uri en_US
dc.source Legacy MARC en_US
dc.subject.other Nitric oxide en_US
dc.subject.other nitric oxide synthase en_US
dc.subject.other fracture healing en_US
dc.subject.other temporal expression en_US
dc.subject.other cellus en_US
dc.title Nitric oxide synthase isoforms during fracture healing en_US
dc.type Journal Article en
dcterms.accessRights metadata only access
dspace.entity.type Publication en_US
unsw.identifier.doiPublisher en_US
unsw.relation.faculty Medicine & Health
unsw.relation.ispartofissue 3 en_US
unsw.relation.ispartofjournal Journal of Bone and Mineral Research en_US
unsw.relation.ispartofpagefrompageto 535-540 en_US
unsw.relation.ispartofvolume 16 en_US
unsw.relation.originalPublicationAffiliation Zhu, Wei en_US
unsw.relation.originalPublicationAffiliation Diwan, Ashish, Clinical School - St George Hospital, Faculty of Medicine, UNSW en_US
unsw.relation.originalPublicationAffiliation Lin, J en_US
unsw.relation.originalPublicationAffiliation Murrell, George, Clinical School - St George Hospital, Faculty of Medicine, UNSW en_US Clinical School St George Hospital *
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