Publication:
Intermolecular complexation and phase separation in aqueous solutions of oppositely charged biopolymers

dc.contributor.author Singh, Salesh en_US
dc.contributor.author Siddhanta, A en_US
dc.contributor.author Meena, R en_US
dc.contributor.author Prasad, K en_US
dc.contributor.author Bandyopadhyay, Srikanta en_US
dc.contributor.author Bohidar, Himadri en_US
dc.date.accessioned 2021-11-25T13:13:02Z
dc.date.available 2021-11-25T13:13:02Z
dc.date.issued 2007 en_US
dc.description.abstract Turbidity measurements performed at 450 nm were used to follow the process of complex formation, and phase separation in gelatin-agar aqueous solutions. Acid (Type-A) and alkali (Type-B) processed gelatin (polyampholyte) and agar (anionic polyelectrolyte) solutions, both having concentration of 0.1% (w/v) were mixed in various proportions, and the mixture was titrated (with 0.01 M HCl or NaOH) to initiate associative complexation that led to coacervation. The titration profiles clearly established observable transitions in terms of the solution pH corresponding to the first occurrence of turbidity (pH(c), formation of soluble complexes), and a point of turbidity maximum (pH(phi), formation of insoluble complexes). Decreasing the pH beyond pH, drove the system towards precipitation. The values of pH(c) and pH(phi) characterized the initiation of the formation of intermolecular charge neutralized soluble aggregates, and the subsequent formation of microscopic coacervate droplets. These aggregates were characterized by dynamic light scattering. It was found that Type-A and -B gelatin samples formed soluble intermolecular complexes (and coacervates) with agar molecules through electrostatic and patch-binding interactions, respectively. (C) 2007 Elsevier B.V. All rights reserved. en_US
dc.identifier.issn 0141-8130 en_US
dc.identifier.uri http://hdl.handle.net/1959.4/39389
dc.language English
dc.language.iso EN en_US
dc.rights CC BY-NC-ND 3.0 en_US
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/3.0/au/ en_US
dc.source Legacy MARC en_US
dc.subject.other intermolecular complexation en_US
dc.subject.other phase diagram en_US
dc.subject.other gelatin-agar en_US
dc.subject.other electrophoresis en_US
dc.subject.other coacervation en_US
dc.title Intermolecular complexation and phase separation in aqueous solutions of oppositely charged biopolymers en_US
dc.type Journal Article en
dcterms.accessRights metadata only access
dspace.entity.type Publication en_US
unsw.accessRights.uri http://purl.org/coar/access_right/c_14cb
unsw.identifier.doiPublisher http://dx.doi.org/10.1016/j.ijbiomac.2007.02.004 en_US
unsw.relation.faculty Science
unsw.relation.ispartofissue 2 en_US
unsw.relation.ispartofjournal International Journal of Biological Macromolecules en_US
unsw.relation.ispartofpagefrompageto 185-192 en_US
unsw.relation.ispartofvolume 41 en_US
unsw.relation.originalPublicationAffiliation Singh, Salesh en_US
unsw.relation.originalPublicationAffiliation Siddhanta, A en_US
unsw.relation.originalPublicationAffiliation Meena, R en_US
unsw.relation.originalPublicationAffiliation Prasad, K en_US
unsw.relation.originalPublicationAffiliation Bandyopadhyay, Srikanta, Materials Science & Engineering, Faculty of Science, UNSW en_US
unsw.relation.originalPublicationAffiliation Bohidar, Himadri, Materials Science & Engineering, Faculty of Science, UNSW en_US
unsw.relation.school School of Materials Science & Engineering *
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