Substance P and ATP activate novel inward currents in myenteric neurons Bertrand, P. P en_US Galligan, J. J. en_US 2021-11-25T13:35:08Z 2021-11-25T13:35:08Z 1992 en_US
dc.description.abstract The actions of substance P (SP), the NK-3 agonist senktide and ATP were studied in single myenteric neurons of guinea pig ileum in vitro. Conventional single electrode voltage clamp was used and responses were compared to those mediated by nicotinic, 5-HT3 and GABAA receptors. Drugs were applied by superfusion or pressure ejection from a fine-tipped pipette. In 8/18 S neurons (RMP= -51 mV, input resistance (IR)= 164 MOhms) pressure applied ATP (1 mM) caused a inward current associated with a increased membrane conductance (gM)(reversal potential (RP) -20 mV). Current latency (< 1s) and decay (< 10s) were similar to nicotinic, 5-HT3 and GABAA responses. Pressure applied SP or senktide (3 mM) caused an inward current in 8/11 AH neurons (RMP= -70 mV, IR= 98 MOhms) of which 2 showed a gM decrease (RP= -98mV), 4 showed a biphasic gM change and 2 showed a gM increase (RP= -9mV). Time course for both responses was .5-1.5 min. (latency: < 1s/gM decrease, > 7s/gM increase). Forskolin (1 mM), caused an inward current which reversed near EK (-98 mV) and selectively occluded the early SP/senktide gM decrease. It is concluded that ATP activates a current similar to that produced by a ligand-gated ion channel, while it is likely that SP/senktide activates a second messenger dependent current. en_US
dc.identifier.issn 0031-7004 en_US
dc.language English
dc.language.iso EN en_US
dc.rights CC BY-NC-ND 3.0 en_US
dc.rights.uri en_US
dc.source Legacy MARC en_US
dc.title Substance P and ATP activate novel inward currents in myenteric neurons en_US
dc.type Journal Article en
dcterms.accessRights metadata only access
dspace.entity.type Publication en_US
unsw.relation.faculty Medicine & Health
unsw.relation.ispartofjournal Pharmacologist en_US
unsw.relation.ispartofpagefrompageto 34-34 en_US
unsw.relation.ispartofvolume 38 en_US
unsw.relation.originalPublicationAffiliation Bertrand, P. P, Medical Sciences, Faculty of Medicine, UNSW en_US
unsw.relation.originalPublicationAffiliation Galligan, J. J. en_US School of Medical Sciences *
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