ATP mediates fast synaptic potentials in enteric neurons Galligan, J. J. en_US Bertrand, P. P en_US 2021-11-25T13:34:47Z 2021-11-25T13:34:47Z 1994 en_US
dc.description.abstract Conventional intracellular electrophysiological methods were used to study fast synaptic transmission in the myenteric plexus of guinea pig ileum in vitro. Fast excitatory postsynaptic potentials (fEPSPs) were evoked in 98 neurons following single stimuli applied to interganglionic connectives. The nicotinic antagonist hexamethonium (100 μM) reduced fEPSPs by 63% in 37 neurons; these fEPSPs were considered to be cholinergic. In 61 neurons, hexamethonium reduced fEPSPs by 33%; fEPSPs recorded in the presence of hexamethonium were considered to be noncholinergic. Similar data were obtained using the nicotinic antagonist mecamylamine (10 μM) to block fEPSPs. Hexamethonium or mecamylamine completely blocked depolarizations caused by acetylcholine (ACh) applied by ionophoresis. The P2 receptor antagonist suramin (1-300 μM) inhibited noncholinergic fEPSPs in 30 cells; the suramin IC50 was 4 μM. Suramin (100 μM) did not block depolarizations caused by ACh or 5-HT, but suramin blocked depolarizations caused by ATP. Hexamethonium did not block ATP-induced depolarizations. The estimated reversal potential for suramin-sensitive fEPSPs and ATP-induced depolarizations was -25 and -16 mV, respectively. ATP responses were reduced in low-sodium (26 mM) extracellular solution, suggesting that ATP activates a cation channel. These data indicate that in myenteric nerves ATP, in addition to ACh, contributes to fast synaptic transmission. en_US
dc.description.uri en_US
dc.identifier.issn 0270-6474 en_US
dc.language English
dc.language.iso EN en_US
dc.rights CC BY-NC-ND 3.0 en_US
dc.rights.uri en_US
dc.source Legacy MARC en_US
dc.title ATP mediates fast synaptic potentials in enteric neurons en_US
dc.type Journal Article en
dcterms.accessRights metadata only access
dspace.entity.type Publication en_US
unsw.relation.faculty Medicine & Health
unsw.relation.ispartofissue 12 en_US
unsw.relation.ispartofjournal Journal of Neuroscience en_US
unsw.relation.ispartofpagefrompageto 7563-7571 en_US
unsw.relation.ispartofvolume 14 en_US
unsw.relation.originalPublicationAffiliation Galligan, J. J. en_US
unsw.relation.originalPublicationAffiliation Bertrand, P. P, Medical Sciences, Faculty of Medicine, UNSW en_US School of Medical Sciences *
Resource type