Publication:
Slow excitatory post-synaptic potentials in myenteric AH neurons of the guinea-pig ileum are reduced by the 5-hydroxytrytamine7 receptor antagonist SB 269970

dc.contributor.author Monro, R. L. en_US
dc.contributor.author Bornstein, J. C. en_US
dc.contributor.author Bertrand, P. P en_US
dc.date.accessioned 2021-11-25T13:35:20Z
dc.date.available 2021-11-25T13:35:20Z
dc.date.issued 2005 en_US
dc.description.abstract Serotonin (5-HT) is a key modulator of neuronal excitability in the central and peripheral nervous system. In the enteric nervous system, 5-HT causes a slow depolarization in the intrinsic sensory neurons, but the receptor responsible for this has not been correlated with known gene products. The aim of this study was to determine whether the newly characterized 5-HT7 receptor may participate in the 5-HT-mediated depolarization of, and synaptic transmission to, the intrinsic sensory neurons of the guinea-pig ileum. Intracellular electrophysiological recordings were made from intrinsic sensory neurons identified as myenteric AH neurons from guinea-pig ileum. 5-HT (5μM) applied to the cell body evoked both a fast depolarization (5-HT3 mediated) and/or a slow depolarization (5-HT1P-like). The 5-HT1/5/7 receptor agonist 5-carboxamidotryptamine (5-CT) (5μM) evoked only a slow depolarization. When the fast depolarization evoked by 5-HT was blocked with granisetron (1μM, 5-HT3 receptor antagonist), only a slow depolarization remained; this was abolished by the 5-HT7 receptor antagonist SB 269970 (1μM, control: 14±2 mV, granisetron+SB 269970: −1±2 mV). The slow depolarization evoked by 5-CT was also significantly reduced by SB 269970 (control: 14±1 mV, SB 269970: 5±2 mV) suggesting a 5-HT7 receptor was activated by exogenous application of 5-CT and 5-HT. Slow excitatory postsynaptic potentials evoked by stimulating descending neural pathways (containing serotonergic fibers) were reduced by SB 269970 (control: 8±3 mV, SB 269970: 3±1 mV). However, SB 269970 had no effect on slow excitatory postsynaptic potentials evoked by stimulation of circumferential (tachykinergic) pathways (control: 7±1 mV, SB 269970: 6±1 mV). These data are consistent with the presence on enteric AH neurons of functional 5-HT7 receptors that participate in slow synaptic transmission. en_US
dc.identifier.issn 0306-4522 en_US
dc.identifier.uri http://hdl.handle.net/1959.4/40048
dc.language English
dc.language.iso EN en_US
dc.rights CC BY-NC-ND 3.0 en_US
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/3.0/au/ en_US
dc.source Legacy MARC en_US
dc.title Slow excitatory post-synaptic potentials in myenteric AH neurons of the guinea-pig ileum are reduced by the 5-hydroxytrytamine7 receptor antagonist SB 269970 en_US
dc.type Journal Article en
dcterms.accessRights metadata only access
dspace.entity.type Publication en_US
unsw.accessRights.uri http://purl.org/coar/access_right/c_14cb
unsw.identifier.doiPublisher http://dx.doi.org/10.1016/j.neuroscience.2005.05.006 en_US
unsw.relation.faculty Medicine & Health
unsw.relation.ispartofjournal Neuroscience en_US
unsw.relation.ispartofpagefrompageto 975-986 en_US
unsw.relation.ispartofvolume 134 en_US
unsw.relation.originalPublicationAffiliation Monro, R. L. en_US
unsw.relation.originalPublicationAffiliation Bornstein, J. C. en_US
unsw.relation.originalPublicationAffiliation Bertrand, P. P, Medical Sciences, Faculty of Medicine, UNSW en_US
unsw.relation.school School of Medical Sciences *
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