The control of copy number of IS6110 in Mycobacterium tuberculosis Tanaka, Mark en_US Rosenberg, N en_US Small, P en_US 2021-11-25T13:26:39Z 2021-11-25T13:26:39Z 2004 en_US
dc.description.abstract Insertion sequence (IS) elements are bacterial genes that are able to transpose to different locations in the genome. These elements are often used in molecular epidemiology as genetic markers that track the spread of pathogens. Transposable elements have frequently been described as `selfish DNA` because they facilitate their own transposition, causing damage when they insert into coding regions, while contributing little if anything to the bacterial host. According to this hypothesis, the expansion of copy number of insertion sequences is opposed by negative selection against high copy numbers. From an alternative point of view, we might expect IS elements to intrinsically regulate transposition within cells, thereby limiting damage to their bacterial host. Here, we report evidence that the copy number of IS6110 in Mycobacterium tuberculosis is controlled by selection against the element. We first construct 12 different models of marker change resulting from a combination of possible transposition functions and selective regimes. We then compute the Akaike Information Criterion for each model to identify the models that best explain data consisting of serial isolates of M. tuberculosis genotyped with IS6110. We find that the best performing models all include selection against the accumulation of copies. Specifically, our analysis points to the interaction of separate copies of the element causing lethal effects. We discuss the implications of these findings for genome evolution and molecular epidemiology. en_US
dc.identifier.issn 0737-4038 en_US
dc.language English
dc.language.iso EN en_US
dc.rights CC BY-NC-ND 3.0 en_US
dc.rights.uri en_US
dc.source Legacy MARC en_US
dc.subject.other Mycobacterium tuberculosis en_US
dc.subject.other molecular epidemiogy en_US
dc.subject.other insertion sequence en_US
dc.subject.other transposition rate en_US
dc.subject.other regulation en_US
dc.subject.other Akaike information criterion en_US
dc.title The control of copy number of IS6110 in Mycobacterium tuberculosis en_US
dc.type Journal Article en
dcterms.accessRights metadata only access
dspace.entity.type Publication en_US
unsw.identifier.doiPublisher en_US
unsw.relation.faculty Science
unsw.relation.ispartofissue 12 en_US
unsw.relation.ispartofjournal Molecular Biology and Evolution en_US
unsw.relation.ispartofpagefrompageto 2195-2201 en_US
unsw.relation.ispartofvolume 21 en_US
unsw.relation.originalPublicationAffiliation Tanaka, Mark, Biotechnology & Biomolecular Sciences, Faculty of Science, UNSW en_US
unsw.relation.originalPublicationAffiliation Rosenberg, N en_US
unsw.relation.originalPublicationAffiliation Small, P en_US School of Biotechnology & Biomolecular Sciences *
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