Intracellular recording from myenteric neurons of the guinea-pig ileum that respond to stretch Kunze, W. A. en_US Furness, J. B. en_US Bertrand, P. P en_US Bornstein, J. C. en_US 2021-11-25T13:34:39Z 2021-11-25T13:34:39Z 1998 en_US
dc.description.abstract 1. Isolated longitudinal muscle-myenteric plexus preparations from guinea-pig ileum were used to investigate the activity of myenteric neurons when the tissue was stretched in the circumferential direction. Membrane potentials were recorded via flexibly mounted intracellular recording electrodes containing Neurobiotin in 1 M KCl. The preparations were stretched to constant widths (+20% and +40% beyond slack width). 2. Multipolar neurons (Dogiel type II morphology) discharged spontaneous action potentials and proximal process potentials during maintained stretching, three of twenty-one at +20% stretch and seven of nine at +40% stretch. At the maximum extent of stretch tried, +40% beyond slack tissue width, action potentials in Dogiel type II neurons occurred at 10-33 Hz. Neurons with other morphologies were all uniaxonal. Some displayed spontaneous fast EPSPs or action potentials, three of forty one at +20% stretch and seven of nineteen at +40% stretch. 3. In seven of eight Dogiel type II neurons, action potentials or proximal process potentials persisted when membrane hyperpolarization was imposed via the recording electrode. Action potential discharge was abolished by hyperpolarization in seven of nine uniaxonal neurons; the exceptions were two orally projecting neurons. 4. Dogiel type II and uniaxonal neurons were classified as rapidly accommodating if they discharged action potentials only at the beginning of a 500 ms intracellular depolarizing pulse and slowly accommodating if they discharged for more than 250 ms. For Dogiel type II neurons, three of thirteen were slowly accommodating at +20% stretch and two of four at 40% stretch. For uniaxonal neurons the corresponding data were twelve of twenty-six and fifteen of nineteen neurons. The slowly accommodating state was associated with increased cell input resistance in uniaxonal neurons. 5. The spontaneous action potential discharge in Dogiel type II and uniaxonal neurons ceased when the muscle was relaxed pharmacologically by nicardipine (3 microM) or isoprenaline (1 microM), although the applied stretch was maintained. At the same time, evoked spike discharge became rapidly accommodating. 6. We conclude that many Dogiel type II neurons, and possibly some orally projecting uniaxonal neurons, are intrinsic, stretch-sensitive, primary afferent neurons that respond to muscle tension with sustained action potential discharge. en_US
dc.description.uri en_US
dc.identifier.issn 0022-3751 en_US
dc.language English
dc.language.iso EN en_US
dc.rights CC BY-NC-ND 3.0 en_US
dc.rights.uri en_US
dc.source Legacy MARC en_US
dc.title Intracellular recording from myenteric neurons of the guinea-pig ileum that respond to stretch en_US
dc.type Journal Article en
dcterms.accessRights metadata only access
dspace.entity.type Publication en_US
unsw.relation.faculty Medicine & Health
unsw.relation.ispartofissue 3 en_US
unsw.relation.ispartofjournal Journal of Physiology en_US
unsw.relation.ispartofpagefrompageto 827-842 en_US
unsw.relation.ispartofvolume 506 en_US
unsw.relation.originalPublicationAffiliation Kunze, W. A. en_US
unsw.relation.originalPublicationAffiliation Furness, J. B. en_US
unsw.relation.originalPublicationAffiliation Bertrand, P. P, Medical Sciences, Faculty of Medicine, UNSW en_US
unsw.relation.originalPublicationAffiliation Bornstein, J. C. en_US School of Medical Sciences *
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