Publication:
Effect of ribosomes and intracellular solutes on activities and stabilities of elongation factor 2 proteins from psychrotolerant and thermophilic methanogens

dc.contributor.author Thomas, Torsten en_US
dc.contributor.author Kumar, N en_US
dc.contributor.author Cavicchioli, R en_US
dc.date.accessioned 2021-11-25T13:24:21Z
dc.date.available 2021-11-25T13:24:21Z
dc.date.issued 2001 en_US
dc.description.abstract Low-temperature-adapted archaea are abundant in the environment, yet little is known about the thermal adaptation of their proteins. We have previously compared elongation factor 2 (EF-2) proteins from Antarctic (Methanococcoides burtonii) and thermophilic (Methanosarcina thermophila) methanogens and found that the M. burtonii EF-2 had greater intrinsic activity at low temperatures and lower thermal stability at high temperatures (T. Thomas and R. Cavicchioli, J. Bacteriol. 182:1328-1332, 2000). While the gross thermal properties correlated with growth temperature, the activity and stability profiles of the EF-2 proteins did not precisely match the optimal growth temperature of each organism. This indicated that intracellular components may affect the thermal characteristics of the EF-2 proteins, and in this study we examined the effects of ribosomes and intracellular solutes. At a high growth temperature the thermophile produced high levels of potassium glutamate, which, when assayed in vitro with EF-2, retarded thermal unfolding and increased catalytic efficiency. In contrast, for the Antarctic methanogen adaptation to growth at a low temperature did not involve the accumulation of stabilizing organic solutes but appeared to result from an increased affinity of EF-2 for GTP and high levels of EF-2 in the cell relative to its low growth rate. Furthermore, ribosomes greatly stimulated GTPase activity and moderately stabilized both EF-2 proteins. These findings illustrate the different physiological strategies that have evolved in two phylogenetically related but thermally distinct methanogens to enable EF-2 to function satisfactorily. en_US
dc.identifier.uri http://hdl.handle.net/1959.4/39564
dc.language English
dc.language.iso EN en_US
dc.rights CC BY-NC-ND 3.0 en_US
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/3.0/au/ en_US
dc.source Legacy MARC en_US
dc.title Effect of ribosomes and intracellular solutes on activities and stabilities of elongation factor 2 proteins from psychrotolerant and thermophilic methanogens en_US
dc.type Journal Article en
dcterms.accessRights metadata only access
dspace.entity.type Publication en_US
unsw.accessRights.uri http://purl.org/coar/access_right/c_14cb
unsw.identifier.doiPublisher http://dx.doi.org/10.1128/JB.183.6.1974-1982.2001 en_US
unsw.relation.faculty Science
unsw.relation.ispartofissue 6 en_US
unsw.relation.ispartofjournal Journal of Bacteriology en_US
unsw.relation.ispartofpagefrompageto 1974-1982 en_US
unsw.relation.ispartofvolume 183 en_US
unsw.relation.originalPublicationAffiliation Thomas, Torsten, Biotechnology & Biomolecular Sciences, Faculty of Science, UNSW en_US
unsw.relation.originalPublicationAffiliation Kumar, N en_US
unsw.relation.originalPublicationAffiliation Cavicchioli, R en_US
unsw.relation.school School of Biotechnology & Biomolecular Sciences *
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