Publication:
In vitro cytotoxicity testing of airborne formaldehyde collected in serum-free culture media

dc.contributor.author Bakand, Shahnaz en_US
dc.contributor.author Hayes, Amanda en_US
dc.contributor.author Winder, Christopher en_US
dc.contributor.author Khalil, Christian en_US
dc.contributor.author Markovic, Boban en_US
dc.date.accessioned 2021-11-25T13:22:30Z
dc.date.available 2021-11-25T13:22:30Z
dc.date.issued 2005 en_US
dc.description.abstract The purpose of this study was to identify a suitable sampling model for on-site toxicity assessment of soluble air contaminants such as formaldehyde, a well known industrial and indoor air contaminant. The in vitro cytotoxicity of formaldehyde, the selected model for soluble air contaminants, was studied using the MTS (tetrazolium salt) assay in two carcinoma cell lines, A549 epithelial lung and HepG2 hepatocarcinoma, and in skin fibroblasts. The cytotoxic effects of airborne formaldehyde were evaluated using test atmospheres in concentrations below 10 ppm (12.3 mg/m(3)), generated by a dynamic diffusion method and bubbled (0.3 L/min) through serum-free culture media for one or four hours. Human cells were treated with formaldehyde air samples, and cell viability was determined after four hours incubation. In parallel, the concentration of airborne formaldehyde was monitored, using the 3500 NIOSH method. Cell viability of the HepG2 cells exposed to formaldehyde air samples (8.75 ppm x 4 h) was reduced to less than 50% (31.6 +/- 1.24%). The HepG2 cell lines were found to be more sensitive (IC50=103.79 +/- 23.55 mg/L) to formaldehyde than both A549 cell lines (IC50 =198.36 +/- 9.54 mg/L) and skin fibroblasts (IC50 = 196.68 +/- 36.73 mg/L) (P < 0.01). An average of 96.8% was determined for collection efficiency of formaldehyde in serum-free culture media. The results of this study suggest that absorption of soluble air contaminants, such as formaldehyde, in serum-free culture media can be used as a suitable sampling model for on-site toxicity assessments. en_US
dc.identifier.issn 0748-2337 en_US
dc.identifier.uri http://hdl.handle.net/1959.4/39507
dc.language English
dc.language.iso EN en_US
dc.rights CC BY-NC-ND 3.0 en_US
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/3.0/au/ en_US
dc.source Legacy MARC en_US
dc.subject.other air contaminants en_US
dc.subject.other cytotoxicity en_US
dc.subject.other formaldehyde en_US
dc.subject.other in vitro toxicity en_US
dc.subject.other MTS (tetrazolium salt) assay en_US
dc.title In vitro cytotoxicity testing of airborne formaldehyde collected in serum-free culture media en_US
dc.type Journal Article en
dcterms.accessRights metadata only access
dspace.entity.type Publication en_US
unsw.accessRights.uri http://purl.org/coar/access_right/c_14cb
unsw.identifier.doiPublisher http://dx.doi.org/10.1191/0748233705th223oa en_US
unsw.relation.faculty Science
unsw.relation.ispartofissue 7-8 en_US
unsw.relation.ispartofjournal Toxicology and Industrial Health en_US
unsw.relation.ispartofpagefrompageto 147-154 en_US
unsw.relation.ispartofvolume 21 en_US
unsw.relation.originalPublicationAffiliation Bakand, Shahnaz, Safety Science, Faculty of Science, UNSW en_US
unsw.relation.originalPublicationAffiliation Hayes, Amanda, Safety Science, Faculty of Science, UNSW en_US
unsw.relation.originalPublicationAffiliation Winder, Christopher, Safety Science, Faculty of Science, UNSW en_US
unsw.relation.originalPublicationAffiliation Khalil, Christian, Safety Science, Faculty of Science, UNSW en_US
unsw.relation.originalPublicationAffiliation Markovic, Boban, Safety Science, Faculty of Science, UNSW en_US
unsw.relation.school School of Risk & Safety Science *
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