Publication:
Extracellular Matrix Composition Significantly Influences Pancreatic Stellate Cell (PSC) Gene Expression Pattern: Role of Transgelin in PSC Function.

dc.contributor.author Apte, Minoti en_US
dc.contributor.author Yang, Lu en_US
dc.contributor.author Phillips, Phoebe en_US
dc.contributor.author Xu, Zhihong en_US
dc.contributor.author Kaplan, Warren en_US
dc.contributor.author Cowley, Mark en_US
dc.date.accessioned 2021-11-25T12:28:25Z
dc.date.available 2021-11-25T12:28:25Z
dc.date.issued 2013 en_US
dc.description.abstract Activated pancreatic stellate cells (PSCs) are responsible for the fibrotic matrix of chronic pancreatitis and pancreatic cancer. In vitro protocols examining PSC biology have usually involved PSCs cultured on plastic, a non-physiological surface. However, PSCs cultured on physiological matrices e.g. MatrigelTM (normal basement membrane) and collagen (fibrotic pancreas), may have distinctly different behaviours compared to cells cultured on plastic. Therefore, we aimed to i) compare PSC gene expression after culture on plastic, MatrigelTM and collagen I; ii) validate the gene array data for transgelin, the most highly dysregulated gene in PSCs grown on activating versus non-activating matrices, at mRNA and protein levels; iii) examine the role of transgelin in PSC function; and iv) assess transgelin expression in human chronic pancreatitis sections. Culture of PSCs on different matrices significantly affected their gene expression pattern. 146, 619 and 432 genes respectively were differentially expressed (p < 0.001) in PSCs cultured on collagen I vs MatrigelTM, MatrigelTM vs plastic and collagen I vs plastic. The highest fold change (12.5 fold upregulation) in gene expression in cells on collagen I vs MatrigelTM, was observed for transgelin (an actin stress fibre associated protein). Transgelin was significantly increased in activated PSCs versus quiescent PSCs. Silencing transgelin expression decreased PSC proliferation and also reduced platelet derived growth factor (PDGF)-induced PSC migration. Notably, transgelin was highly expressed in chronic pancreatitis in stromal areas and peri-acinar spaces but was absent in acinar cells. These findings suggest that transgelin is a potentially useful target protein to modulate PSC function so as to ameliorate pancreatic fibrosis. en_US
dc.identifier.issn 0002-9513 en_US
dc.identifier.uri http://hdl.handle.net/1959.4/53263
dc.language English
dc.language.iso EN en_US
dc.rights CC BY-NC-ND 3.0 en_US
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/3.0/au/ en_US
dc.source Legacy MARC en_US
dc.subject.other pancreatic stellate cells en_US
dc.subject.other transgelin en_US
dc.title Extracellular Matrix Composition Significantly Influences Pancreatic Stellate Cell (PSC) Gene Expression Pattern: Role of Transgelin in PSC Function. en_US
dc.type Journal Article en
dcterms.accessRights metadata only access
dspace.entity.type Publication en_US
unsw.accessRights.uri http://purl.org/coar/access_right/c_14cb
unsw.identifier.doiPublisher http://dx.doi.org/10.1152/ajpgi.00016.2013 en_US
unsw.relation.faculty Medicine & Health
unsw.relation.ispartofissue 6 en_US
unsw.relation.ispartofjournal American Journal of Physiology en_US
unsw.relation.ispartofpagefrompageto G408-G417 en_US
unsw.relation.ispartofvolume 305 en_US
unsw.relation.originalPublicationAffiliation Apte, Minoti, Clinical School - South Western Sydney, Faculty of Medicine, UNSW en_US
unsw.relation.originalPublicationAffiliation Yang, Lu, Clinical School - South Western Sydney, Faculty of Medicine, UNSW en_US
unsw.relation.originalPublicationAffiliation Phillips, Phoebe, Children's Cancer Institute Australia for Medical Research, Faculty of Medicine, UNSW en_US
unsw.relation.originalPublicationAffiliation Xu, Zhihong, Clinical School - South Western Sydney, Faculty of Medicine, UNSW en_US
unsw.relation.originalPublicationAffiliation Kaplan, Warren, Garvan Institute of Medical Research, Faculty of Medicine, UNSW en_US
unsw.relation.originalPublicationAffiliation Cowley, Mark, Garvan Institute of Medical Research, Faculty of Medicine, UNSW en_US
unsw.relation.school Clinical School South West Sydney Area Health Service *
unsw.relation.school Childhood Cancer Research *
unsw.relation.school Garvan Institute *
unsw.subject.fieldofresearchcode 110307 Gastroenterology and Hepatology en_US
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