Publication:
BMP-13 Emerges as a Potential Inhibitor for bone formation
BMP-13 Emerges as a Potential Inhibitor for bone formation
dc.contributor.author | Shen, Bojiang | en_US |
dc.contributor.author | Bhargav, Divya | en_US |
dc.contributor.author | Wei, Ai-Qun | en_US |
dc.contributor.author | Williams, Lisa | en_US |
dc.contributor.author | Diwan, Ashish | en_US |
dc.date.accessioned | 2021-11-25T15:32:08Z | |
dc.date.available | 2021-11-25T15:32:08Z | |
dc.date.issued | 2009 | en_US |
dc.description.abstract | Bone morphogenetic protein-13 (BMP-13) plays an important role in skeletal development. In the light of a recent report that mutations in the BMP-13 gene are associated with spine vertebral fusion in Klippel-Feil syndrome, we hypothesized that BMP-13 signaling is crucial for regulating embryonic endochondral ossification. In this study, we found that BMP-13 inhibited the osteogenic differentiation of human bone marrow multipotent mesenchymal stromal cells (BM MSCs) in vitro. The endogenous BMP-13 gene expression in MSCs was examined under expansion conditions. The MSCs were then induced to differentiate into osteoblasts in osteo-inductive medium containing exogenous BMP-13. Gene expression was analysed by real-time PCR. Alkaline phosphatase (ALP) expression and activity, proteoglycan (PG) synthesis and matrix mineralization were assessed by cytological staining or ALP assay. Results showed that endogenous BMP-13 mRNA expression was higher than BMP-2 or -7 during MSC growth. BMP-13 supplementation strongly inhibited matrix mineralization and ALP activity of osteogenic differentiated MSCs, yet increased PG synthesis under the same conditions. In conclusion, BMP-13 inhibited osteogenic differentiation of MSCs, implying that functional mutations or deficiency of BMP-13 may allow excess bone formation. Our finding provides an insight into the molecular mechanisms and the therapeutic potential of BMP-13 in restricting pathological bone formation. | en_US |
dc.description.uri | http://www.biolsci.org/v05p0192.htm | en_US |
dc.identifier.issn | 1449-2288 | en_US |
dc.identifier.uri | http://hdl.handle.net/1959.4/44688 | |
dc.language | English | |
dc.language.iso | EN | en_US |
dc.rights | CC BY-NC-ND 3.0 | en_US |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/3.0/au/ | en_US |
dc.source | Legacy MARC | en_US |
dc.subject.other | BMP13 | en_US |
dc.subject.other | Intervertebral disc degeneration | en_US |
dc.subject.other | Regeneration | en_US |
dc.subject.other | Histological | en_US |
dc.subject.other | In vivo | en_US |
dc.subject.other | Ovine model | en_US |
dc.subject.other | Annular injury | en_US |
dc.subject.other | Nucleus | en_US |
dc.title | BMP-13 Emerges as a Potential Inhibitor for bone formation | en_US |
dc.type | Journal Article | en |
dcterms.accessRights | open access | |
dspace.entity.type | Publication | en_US |
unsw.accessRights.uri | https://purl.org/coar/access_right/c_abf2 | |
unsw.description.publisherStatement | Copyright ©2010 Ivyspring International Publisher. | en_US |
unsw.relation.faculty | Other UNSW | |
unsw.relation.faculty | Medicine & Health | |
unsw.relation.ispartofissue | 2 | en_US |
unsw.relation.ispartofjournal | International Journal of biological Sciences | en_US |
unsw.relation.ispartofpagefrompageto | 192-200 | en_US |
unsw.relation.ispartofvolume | 5 | en_US |
unsw.relation.originalPublicationAffiliation | Shen, Bojiang, UNSW | en_US |
unsw.relation.originalPublicationAffiliation | Bhargav, Divya, Clinical School - St George Hospital, Faculty of Medicine, UNSW | en_US |
unsw.relation.originalPublicationAffiliation | Wei, Ai-Qun | en_US |
unsw.relation.originalPublicationAffiliation | Williams, Lisa, Clinical School - St George Hospital, Faculty of Medicine, UNSW | en_US |
unsw.relation.originalPublicationAffiliation | Diwan, Ashish, Clinical School - St George Hospital, Faculty of Medicine, UNSW | en_US |
unsw.relation.school | Clinical School St George Hospital | * |