Publication:
Changes in sphingomyelin level affect alpha-synuclein and ABCA5 expression

dc.contributor.author Woojin, Scott Kim en_US
dc.contributor.author Halliday, Glenda en_US
dc.date.accessioned 2021-11-25T12:28:57Z
dc.date.available 2021-11-25T12:28:57Z
dc.date.issued 2012 en_US
dc.description.abstract The pathological hallmark of Parkinson’s disease (PD) is the presence of aggregated alpha-synuclein associated with lipids. Substantial evidence now exists to indicate that alpha-synuclein binds and interacts with regions of membranes that are enriched in lipids (lipid rafts). Lipids are transported around the brain by a group of proteins called ATP-Binding Cassette subfamily A (ABCA) transporters and in recent years there has been mounting evidence indicating that ABCA transporters regulate a number of neurodegenerative disease processes. Only recently a genome-wide association study reported that ABCA5 was genetically associated with a reduced risk for PD. However, very little is known about the role of lipids and ABCA5 in the pathological process of PD. In this study we investigate the impact of the lipid-raft lipid sphingomyelin on alpha-synuclein andABCA5expression. We also investigate in which primary human brain cells ABCA5 is expressed and whether the expression of ABCA5 is altered in PD brain. When SK-N-SH neuronal cells were treated with sphingomyelin the expression of both -synuclein and ABCA5 was significantly increased, indicating sphingomyelin as a potential substrate for the ABCA5 transporter. ABCA5 was strongly expressed in neurons and moderately in microglia, with only weak expression observed in astrocytes and oligodendrocytes. The expression of ABCA5 was significantly elevated in PD brains compared to age- and gender-matched control brains, possibly as a protective response to the disease. These data provide new evidence indicating that lipid is important for alpha-synuclein pathology in PD. en_US
dc.identifier.issn 1877-7171 en_US
dc.identifier.uri http://hdl.handle.net/1959.4/53439
dc.language English
dc.language.iso EN en_US
dc.rights CC BY-NC-ND 3.0 en_US
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/3.0/au/ en_US
dc.source Legacy MARC en_US
dc.subject.other ABCA5 en_US
dc.subject.other alpha-synuclein en_US
dc.subject.other sphingomyelin en_US
dc.subject.other lipid en_US
dc.subject.other neuron en_US
dc.subject.other neurodegeneration en_US
dc.title Changes in sphingomyelin level affect alpha-synuclein and ABCA5 expression en_US
dc.type Journal Article en
dcterms.accessRights metadata only access
dspace.entity.type Publication en_US
unsw.accessRights.uri http://purl.org/coar/access_right/c_14cb
unsw.identifier.doiPublisher http://dx.doi.org/10.3233/JPD-2012-11059 en_US
unsw.relation.FunderRefNo GNT1008307 en_US
unsw.relation.FunderRefNoURL http://purl.org/au-research/grants/nhmrc/1008307 en_US
unsw.relation.faculty Medicine & Health
unsw.relation.fundingScheme NHMRC Project en_US
unsw.relation.ispartofissue 1 en_US
unsw.relation.ispartofjournal Journal of Parkinson's disease en_US
unsw.relation.ispartofpagefrompageto 41-46 en_US
unsw.relation.ispartofvolume 2 en_US
unsw.relation.originalPublicationAffiliation Woojin, Scott Kim, Neuroscience Research Australia, Faculty of Medicine, UNSW en_US
unsw.relation.originalPublicationAffiliation Halliday, Glenda, Neuroscience Research Australia, Faculty of Medicine, UNSW en_US
unsw.relation.school Neuroscience Research Australia *
unsw.subject.fieldofresearchcode 110903 Central Nervous System en_US
Files
Resource type