Publication:
Real-time detection of serotonin release from enterochromaffin cells of the guinea pig ileum
Real-time detection of serotonin release from enterochromaffin cells of the guinea pig ileum
dc.contributor.author | Bertrand, P. P | en_US |
dc.date.accessioned | 2021-11-25T13:34:42Z | |
dc.date.available | 2021-11-25T13:34:42Z | |
dc.date.issued | 2004 | en_US |
dc.description.abstract | Serotonin (5-hydroxytryptamine; 5-HT) containing enterochromaffin (EC) cells may detect chemical or mechanical stimuli in the intestinal lumen and respond with release of 5-HT. The aim of this study was to use real-time electrochemical detection methods to detect release of 5-HT from small numbers of EC cells. In guinea-pig ileum, basal release of 5-HT from the unstimulated, unparalyzed intestine was composed of individual release events (8.4 ± 1.8 events, 0.33 ± 0.06 Hz) of different amplitudes but with similar kinetics. Local compression of the mucosa with the electrode evoked peak 5-HT release of 12.3 ± 2.8 μmol L−1 with a sustained release of 3.0 ± 0.7 μmol L−1. Brief application of acetylcholine (ACh) or carbachol elicited a transient peak (5.7 ± 1.3 μmol L−1 occurring at 35 ± 18 s, n = 9) followed by cyclic release of 5-HT (9.7 ± 2.2 events, 0.40 ± 0.13 Hz, n = 6). This study shows that the release of 5-HT occurs rapidly as individual events from a small number of cells and can reach very high concentrations locally. | en_US |
dc.identifier.issn | 1365-2982 | en_US |
dc.identifier.uri | http://hdl.handle.net/1959.4/40016 | |
dc.language | English | |
dc.language.iso | EN | en_US |
dc.rights | CC BY-NC-ND 3.0 | en_US |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/3.0/au/ | en_US |
dc.source | Legacy MARC | en_US |
dc.title | Real-time detection of serotonin release from enterochromaffin cells of the guinea pig ileum | en_US |
dc.type | Journal Article | en |
dcterms.accessRights | metadata only access | |
dspace.entity.type | Publication | en_US |
unsw.accessRights.uri | http://purl.org/coar/access_right/c_14cb | |
unsw.identifier.doiPublisher | http://dx.doi.org/10.1111/j.1365-2982.2004.00572.x | en_US |
unsw.relation.faculty | Medicine & Health | |
unsw.relation.ispartofjournal | Neurogastroenterology & Motility | en_US |
unsw.relation.ispartofpagefrompageto | 511-514 | en_US |
unsw.relation.ispartofvolume | 16 | en_US |
unsw.relation.originalPublicationAffiliation | Bertrand, P. P, Medical Sciences, Faculty of Medicine, UNSW | en_US |
unsw.relation.school | School of Medical Sciences | * |