Publication:
Real-time detection of serotonin release from enterochromaffin cells of the guinea pig ileum

dc.contributor.author Bertrand, P. P en_US
dc.date.accessioned 2021-11-25T13:34:42Z
dc.date.available 2021-11-25T13:34:42Z
dc.date.issued 2004 en_US
dc.description.abstract Serotonin (5-hydroxytryptamine; 5-HT) containing enterochromaffin (EC) cells may detect chemical or mechanical stimuli in the intestinal lumen and respond with release of 5-HT. The aim of this study was to use real-time electrochemical detection methods to detect release of 5-HT from small numbers of EC cells. In guinea-pig ileum, basal release of 5-HT from the unstimulated, unparalyzed intestine was composed of individual release events (8.4 ± 1.8 events, 0.33 ± 0.06 Hz) of different amplitudes but with similar kinetics. Local compression of the mucosa with the electrode evoked peak 5-HT release of 12.3 ± 2.8 μmol L−1 with a sustained release of 3.0 ± 0.7 μmol L−1. Brief application of acetylcholine (ACh) or carbachol elicited a transient peak (5.7 ± 1.3 μmol L−1 occurring at 35 ± 18 s, n = 9) followed by cyclic release of 5-HT (9.7 ± 2.2 events, 0.40 ± 0.13 Hz, n = 6). This study shows that the release of 5-HT occurs rapidly as individual events from a small number of cells and can reach very high concentrations locally. en_US
dc.identifier.issn 1365-2982 en_US
dc.identifier.uri http://hdl.handle.net/1959.4/40016
dc.language English
dc.language.iso EN en_US
dc.rights CC BY-NC-ND 3.0 en_US
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/3.0/au/ en_US
dc.source Legacy MARC en_US
dc.title Real-time detection of serotonin release from enterochromaffin cells of the guinea pig ileum en_US
dc.type Journal Article en
dcterms.accessRights metadata only access
dspace.entity.type Publication en_US
unsw.accessRights.uri http://purl.org/coar/access_right/c_14cb
unsw.identifier.doiPublisher http://dx.doi.org/10.1111/j.1365-2982.2004.00572.x en_US
unsw.relation.faculty Medicine & Health
unsw.relation.ispartofjournal Neurogastroenterology & Motility en_US
unsw.relation.ispartofpagefrompageto 511-514 en_US
unsw.relation.ispartofvolume 16 en_US
unsw.relation.originalPublicationAffiliation Bertrand, P. P, Medical Sciences, Faculty of Medicine, UNSW en_US
unsw.relation.school School of Medical Sciences *
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