Publication:
Serotonin (5-HT) release and uptake measured by real-time electrochemical techniques in the rat ileum
Serotonin (5-HT) release and uptake measured by real-time electrochemical techniques in the rat ileum
dc.contributor.author | Bertrand, P. P | en_US |
dc.contributor.author | Hu, X. | en_US |
dc.contributor.author | Mach, J. | en_US |
dc.contributor.author | Bertrand, R. L. | en_US |
dc.date.accessioned | 2021-11-25T13:34:54Z | |
dc.date.available | 2021-11-25T13:34:54Z | |
dc.date.issued | 2008 | en_US |
dc.description.abstract | Serotonin (5-HT) is released from the enterochromaffin cells and plays an important role in regulating intestinal function. Although the release of 5-HT is well documented, the contribution of the serotonin reuptake transporter (SERT) to the levels and actions of 5-HT in the intestine is unclear. This study aimed to demonstrate real-time SERT activity in ileal mucosa and to assess the effects of SERT inhibition using fluoxetine. Electrochemical recordings were made from the mucosa in full-thickness preparations of rat ileum using a carbon fiber electrode to measure 5-HT oxidation current and a force transducer to record circular muscle (CM) tension. Compression of the mucosa stimulated a peak 5-HT release of 12 ± 6 µM, which decayed to 7 ± 4 µM. Blockade of SERT with fluoxetine (1 µM) increased the peak compression-evoked release to 19 ± 9 µM, and the background levels of 5-HT increased to 11 ± 7 µM (P < 0.05, n = 7). When 5-HT was exogenously applied to the mucosa, fluoxetine caused a significant increase in the time to 50% and 80% decay of the oxidation current. Fluoxetine also increased the spontaneous CM motility (P < 0.05; n = 7) but did not increase the CM contraction-evoked 5-HT release (P > 0.05, n = 5). In conclusion, this is the first characterization of the real-time uptake of 5-HT into the rat intestine. These data suggest that SERT plays an important role in the modulation of 5-HT concentrations that reach intestinal 5-HT receptors. | en_US |
dc.identifier.issn | 0193-1857 | en_US |
dc.identifier.uri | http://hdl.handle.net/1959.4/40026 | |
dc.language | English | |
dc.language.iso | EN | en_US |
dc.rights | CC BY-NC-ND 3.0 | en_US |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/3.0/au/ | en_US |
dc.source | Legacy MARC | en_US |
dc.title | Serotonin (5-HT) release and uptake measured by real-time electrochemical techniques in the rat ileum | en_US |
dc.type | Journal Article | en |
dcterms.accessRights | metadata only access | |
dspace.entity.type | Publication | en_US |
unsw.accessRights.uri | http://purl.org/coar/access_right/c_14cb | |
unsw.identifier.doiPublisher | http://dx.doi.org/10.1152/ajpgi.90375.2008 | en_US |
unsw.relation.faculty | Medicine & Health | |
unsw.relation.ispartofissue | 6 | en_US |
unsw.relation.ispartofjournal | American Journal of Physiology | en_US |
unsw.relation.ispartofpagefrompageto | G1228-G1236 | en_US |
unsw.relation.ispartofvolume | 295 | en_US |
unsw.relation.originalPublicationAffiliation | Bertrand, P. P, Medical Sciences, Faculty of Medicine, UNSW | en_US |
unsw.relation.originalPublicationAffiliation | Hu, X. | en_US |
unsw.relation.originalPublicationAffiliation | Mach, J. | en_US |
unsw.relation.originalPublicationAffiliation | Bertrand, R. L. | en_US |
unsw.relation.school | School of Medical Sciences | * |