Publication:
Characterization of Thiobacillus thioparus isolated from an activated sludge used for hydrogen sulfide treatment
Characterization of Thiobacillus thioparus isolated from an activated sludge used for hydrogen sulfide treatment
dc.contributor.author | Burgess, J | en_US |
dc.contributor.author | Stuetz, Richard | en_US |
dc.contributor.author | Barbosa, V | en_US |
dc.contributor.author | Atkins, S | en_US |
dc.contributor.author | Barbosa, V | en_US |
dc.date.accessioned | 2021-11-25T13:49:46Z | |
dc.date.available | 2021-11-25T13:49:46Z | |
dc.date.issued | 2006 | en_US |
dc.description.abstract | Aims: To compare Thiobacillus thioparus population dynamics in a control and a test activated sludge (AS) bioreactor, used for hydrogen sulfide (H2S) degradation. Methods and Results: Denaturing gradient gel electrophoresis (DGGE) was used to confirm the presence of T. thioparus, and real-time PCR was used to quantify the level of this bacterium in the AS samples. The DGGE analysis showed a band for T. thioparus in all samples, with the band being more prominent in the test sample with H2S diffusion. It also showed that although a change occurred in the diversity of the microbial population in the test sludge after 6 weeks of H2S diffusion, the microbial community structure of the test and control was still similar. Thiobacillus thioparus-specific PCR primers confirmed that 50% of the isolates from both the test and control bioreactors were T. thioparus. The thiobacilli population became more efficient at degrading the diffused H2S. This increase in efficiency was confirmed by a significant increase in the number of isolates from the test sludge compared with those from the control sludge, when they were grown in a thiosulfate-rich liquid medium. Conclusions: It was concluded that the use of AS process for H2S removal encourages the population of T. thioparus to increase even at times when the total biomass concentration shows a decrease. Significance and Impact of the Study: The research results give an insight into the dynamics of the microbial population in an AS pilot plant used in a dual role, to treat the wastewater and H2S. | en_US |
dc.identifier.issn | 1364-5072 | en_US |
dc.identifier.uri | http://hdl.handle.net/1959.4/41196 | |
dc.language | English | |
dc.language.iso | EN | en_US |
dc.rights | CC BY-NC-ND 3.0 | en_US |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/3.0/au/ | en_US |
dc.source | Legacy MARC | en_US |
dc.title | Characterization of Thiobacillus thioparus isolated from an activated sludge used for hydrogen sulfide treatment | en_US |
dc.type | Journal Article | en |
dcterms.accessRights | metadata only access | |
dspace.entity.type | Publication | en_US |
unsw.accessRights.uri | http://purl.org/coar/access_right/c_14cb | |
unsw.identifier.doiPublisher | http://dx.doi.org/10.1111/j.1365-2672.2006.03032.x | en_US |
unsw.relation.faculty | Engineering | |
unsw.relation.ispartofissue | No.6 | en_US |
unsw.relation.ispartofjournal | Journal of Applied Microbiology | en_US |
unsw.relation.ispartofpagefrompageto | 1269-1281 | en_US |
unsw.relation.ispartofvolume | 101 | en_US |
unsw.relation.originalPublicationAffiliation | Burgess, J | en_US |
unsw.relation.originalPublicationAffiliation | Stuetz, Richard, Civil & Environmental Engineering, Faculty of Engineering, UNSW | en_US |
unsw.relation.originalPublicationAffiliation | Barbosa, V | en_US |
unsw.relation.originalPublicationAffiliation | Atkins, S | en_US |
unsw.relation.originalPublicationAffiliation | Barbosa, V | en_US |
unsw.relation.school | School of Civil and Environmental Engineering | * |