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Generation of mice deficient in both KLF3/BKLF and KLF8 reveals a genetic interaction and a role for these factors in embryonic globin gene silencing

dc.contributor.author Funnell, Alister en_US
dc.contributor.author Mak, Ka Sin en_US
dc.contributor.author Twine, Natalie en_US
dc.contributor.author Pelka, G en_US
dc.contributor.author Norton, Laura en_US
dc.contributor.author Radziewic, T en_US
dc.contributor.author Power, M en_US
dc.contributor.author Wilkins, Marc en_US
dc.contributor.author Bell Anderson, Kim en_US
dc.contributor.author Fraser, Stuart en_US
dc.contributor.author Perkins, Andrew en_US
dc.contributor.author Tam, P en_US
dc.contributor.author Pearson, Richard en_US
dc.contributor.author Crossley, Merlin en_US
dc.date.accessioned 2021-11-25T12:27:40Z
dc.date.available 2021-11-25T12:27:40Z
dc.date.issued 2013 en_US
dc.description.abstract Krüppel-like factors 3 and 8 (KLF3 and KLF8) are highly related transcriptional regulators that bind to similar sequences of DNA. We have previously shown that in erythroid cells there is a regulatory hierarchy within the KLF family, whereby KLF1 drives the expression of both the Klf3 and Klf8 genes and KLF3 in turn represses Klf8 expression. While the erythroid roles of KLF1 and KLF3 have been explored, the contribution of KLF8 to this regulatory network has been unknown. To investigate this, we have generated a mouse model with disrupted KLF8 expression. While these mice are viable, albeit with a reduced life span, mice lacking both KLF3 and KLF8 die at around embryonic day 14.5 (E14.5), indicative of a genetic interaction between these two factors. In the fetal liver, Klf3 Klf8 double mutant embryos exhibit greater dysregulation of gene expression than either of the two single mutants. In particular, we observe derepression of embryonic, but not adult, globin expression. Taken together, these results suggest that KLF3 and KLF8 have overlapping roles in vivo and participate in the silencing of embryonic globin expression during development. en_US
dc.identifier.issn 0270-7306 en_US
dc.identifier.uri http://hdl.handle.net/1959.4/53159
dc.language English
dc.language.iso EN en_US
dc.rights CC BY-NC-ND 3.0 en_US
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/3.0/au/ en_US
dc.source Legacy MARC en_US
dc.subject.other Transcriptional regulation en_US
dc.subject.other KLF3 en_US
dc.subject.other KLF8 en_US
dc.title Generation of mice deficient in both KLF3/BKLF and KLF8 reveals a genetic interaction and a role for these factors in embryonic globin gene silencing en_US
dc.type Journal Article en
dcterms.accessRights open access
dspace.entity.type Publication en_US
unsw.accessRights.uri https://purl.org/coar/access_right/c_abf2
unsw.identifier.doiPublisher http://dx.doi.org/10.1128/MCB.00074-13 en_US
unsw.relation.faculty Science
unsw.relation.ispartofissue 15 en_US
unsw.relation.ispartofjournal Molecular and Cellular Biology en_US
unsw.relation.ispartofpagefrompageto 2976-2987 en_US
unsw.relation.ispartofvolume 33 en_US
unsw.relation.originalPublicationAffiliation Funnell, Alister, Biotechnology & Biomolecular Sciences, Faculty of Science, UNSW en_US
unsw.relation.originalPublicationAffiliation Mak, Ka Sin, Biotechnology & Biomolecular Sciences, Faculty of Science, UNSW en_US
unsw.relation.originalPublicationAffiliation Twine, Natalie, Biotechnology & Biomolecular Sciences, Faculty of Science, UNSW en_US
unsw.relation.originalPublicationAffiliation Pelka, G en_US
unsw.relation.originalPublicationAffiliation Norton, Laura, Biotechnology & Biomolecular Sciences, Faculty of Science, UNSW en_US
unsw.relation.originalPublicationAffiliation Radziewic, T en_US
unsw.relation.originalPublicationAffiliation Power, M en_US
unsw.relation.originalPublicationAffiliation Wilkins, Marc, Biotechnology & Biomolecular Sciences, Faculty of Science, UNSW en_US
unsw.relation.originalPublicationAffiliation Bell Anderson, Kim en_US
unsw.relation.originalPublicationAffiliation Fraser, Stuart en_US
unsw.relation.originalPublicationAffiliation Perkins, Andrew en_US
unsw.relation.originalPublicationAffiliation Tam, P en_US
unsw.relation.originalPublicationAffiliation Pearson, Richard, Biotechnology & Biomolecular Sciences, Faculty of Science, UNSW en_US
unsw.relation.originalPublicationAffiliation Crossley, Merlin, Biotechnology & Biomolecular Sciences, Faculty of Science, UNSW en_US
unsw.relation.school School of Biotechnology & Biomolecular Sciences *
unsw.subject.fieldofresearchcode 060199 Biochemistry and Cell Biology not elsewhere classified en_US
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