ESI-MS and Thermal Melting Studies of Nanoscale Metallomacrocycles with DNA Schilter, David en_US Uramthamakul, T en_US Beck, Jenny en_US Harding, Margaret en_US Rendina, Louis en_US 2021-11-25T16:44:04Z 2021-11-25T16:44:04Z 2010 en_US
dc.description.abstract The hydrophilic, long-chain diamine PEGda (O,O′-bis(2-aminoethyl)octadeca(ethylene glycol)), when complexed with cis-protected Pt(II) ions afforded water-soluble complexes of the general type [Pt(N,N)(PEGda)](NO3)2 (N,N = N,N,N′,N′-tetramethyl-1,2-diaminoethane (tmeda), 1,2-diaminoethane (en), and 2,2′-bipyridine (2,2′-bipy)) featuring unusual 62 membered chelate rings. Equimolar mixtures containing either the 16-mer duplex DNA D2 or the single-stranded D2a and [Pt(N,N)(PEGda)]2+ were analyzed by negative-ion ESI MS. Analysis of D2-Pt(II) mixtures showed the formation of 1:1 adducts of [Pt(en)(PEGda)]2+, [Pt(tmeda)(PEGda)]2+ and the previously-described metallomacrocycle [Pt2(2,2′-bipy)2{4,4′-bipy(CH2)44,4′-bipy}2]8+ with D2; the dinuclear species bound to D2 most strongly, consistent with its greater charge and aryl surface area. The non-macrocyclic species [Pt(2,2′-bipy)(Mebipy)2]4+ and [Pt(2,2′ bipy)(NH3)2]2+ formed 1: 2 complexes with D2. Analyses of D2a-Pt(II) mixtures gave results similar to those obtained with D2, although fragmentation was more pronounced, indicating that the nucleobases in D2a play more significant roles in mediating the decomposition of complexes than those in D2, in which they are paired in a complementary manner. Investigations were also conducted into the effects of selected platinum(II) complexes on the thermal denaturation of calf-thymus DNA (CT-DNA) in buffered solution. Both [Pt2(2,2′ bipy)2{4,4′-bipy(CH2)64,4′-bipy}2]8+ and [Pt(2,2′-bipy)(Mebipy)2]4+ stabilized CT-DNA. In contrast, [Pt(tmeda)(PEGda)]2+ and [Pt(en)(PEGda)]2+ (as well as free PEGda) caused negligible changes in melting temperature (∆Tm), suggesting that these species interact weakly with CT-DNA. Similar ∆Tm values for [Pt(2,2′ bipy)(PEGda)]2+ and [Pt(2,2′-bipy)(NH3)2]2+ imply that PEGda does not play a major role in DNA binding. en_US
dc.identifier.issn 1477-9226 en_US
dc.language English
dc.language.iso EN en_US
dc.rights CC BY-NC-ND 3.0 en_US
dc.rights.uri en_US
dc.source Legacy MARC en_US
dc.subject.other Inorganic Chemistry (030200) en_US
dc.subject.other Medicinal and Biomolecular Chemistry (030400) en_US
dc.title ESI-MS and Thermal Melting Studies of Nanoscale Metallomacrocycles with DNA en_US
dc.type Journal Article en
dcterms.accessRights metadata only access
dspace.entity.type Publication en_US
unsw.identifier.doiPublisher en_US
unsw.relation.faculty Science
unsw.relation.ispartofjournal Journal of the Royal Chemical Society, Dalton Transactions en_US
unsw.relation.ispartofpagefrompageto 11263-11271 en_US
unsw.relation.ispartofvolume 39 en_US
unsw.relation.originalPublicationAffiliation Schilter, David en_US
unsw.relation.originalPublicationAffiliation Uramthamakul, T en_US
unsw.relation.originalPublicationAffiliation Beck, Jenny en_US
unsw.relation.originalPublicationAffiliation Harding, Margaret, Chemistry, Faculty of Science, UNSW en_US
unsw.relation.originalPublicationAffiliation Rendina, Louis en_US School of Chemistry *
Resource type