Publication:
Real-time measurement of serotonin release and motility in guinea pig ileum

dc.contributor.author Bertrand, P. P en_US
dc.date.accessioned 2021-11-25T13:35:59Z
dc.date.available 2021-11-25T13:35:59Z
dc.date.issued 2006 en_US
dc.description.abstract Enterochromaffin (EC) cells are sensors that detect chemical or mechanical stimuli and respond with release of serotonin (5-HT). 5-HT activates local motor reflexes, but whether local motor reflexes also evoke 5-HT release is unknown. The aim of the present study was to establish the relationship between the release of 5-HT and the enteric neural circuits controlling the movements of the intestine. Recordings were made from full-thickness preparations of guinea pig ileum using electrochemical techniques with carbon fibre electrodes to measure local concentrations of 5-HT. The tension in the circular muscle (CM) and longitudinal muscle (LM) was recorded with force transducers. The release of 5-HT from the EC cells was detected selectively and the timing of the events quantified. Pressure-evoked peristalsis caused detectable 5-HT release only when the recording site was invaded by a ring of CM contraction. Spontaneous and stretch-evoked reflex contraction of the CM and LM occurred simultaneously with 5-HT release. Paralysis of the smooth muscle significantly reduced the stretch-evoked release. Muscarinic agonists evoked reflexes that were associated with increases in tension in CM and LM simultaneous with 5-HT release. Tetrodotoxin abolished the coordination between the CM contraction and 5-HT release but not the direct activation of the CM and EC cells by the agonists. In conclusion, the correlation between local motor reflexes and 5-HT release observed in the present study is caused primarily by the contraction of the smooth muscle and subsequent deformation of the mucosa. The EC cell is, thus, a site of convergence for mechanical forces that contribute to the release of 5-HT during motor reflexes. en_US
dc.identifier.issn 0022-3751 en_US
dc.identifier.uri http://hdl.handle.net/1959.4/40077
dc.language English
dc.language.iso EN en_US
dc.rights CC BY-NC-ND 3.0 en_US
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/3.0/au/ en_US
dc.source Legacy MARC en_US
dc.title Real-time measurement of serotonin release and motility in guinea pig ileum en_US
dc.type Journal Article en
dcterms.accessRights open access
dspace.entity.type Publication en_US
unsw.accessRights.uri https://purl.org/coar/access_right/c_abf2
unsw.description.publisherStatement The definitive version is available at www3.interscience.wiley.com en_US
unsw.identifier.doiPublisher http://dx.doi.org/10.1113/jphysiol.2006.117804 en_US
unsw.relation.faculty Medicine & Health
unsw.relation.ispartofjournal Journal of Physiology en_US
unsw.relation.ispartofpagefrompageto 689-704 en_US
unsw.relation.ispartofvolume 577 en_US
unsw.relation.originalPublicationAffiliation Bertrand, P. P, Medical Sciences, Faculty of Medicine, UNSW en_US
unsw.relation.school School of Medical Sciences *
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