A novel in vitro exposure technique for toxicity testing of selected volatile organic compounds Bakand, S. en_US Winder, C. en_US Khalil, Christian en_US Hayes, A. en_US 2021-11-25T13:25:08Z 2021-11-25T13:25:08Z 2006 en_US
dc.description.abstract Exposure to vapours of volatile chemicals is a major occupational and environmental health concern. Toxicity testing of volatile organic compounds (VOCs) has always faced significant technological problems due to their high volatility and/or low solubility. The aim of this study was to develop a practical and reproducible in vitro exposure technique for toxicity testing of VOCs. Standard test atmospheres of xylene and toluene were generated in glass chambers using a static method. Human cells including: A549-lung derived cell lines, HepG2-liver derived cell lines and skin fibroblasts, were grown in porous membranes and exposed to various airborne concentrations of selected VOCs directly at the air/liquid interface for 1 h at 37 degrees C. Cytotoxicity of test chemicals was investigated using the MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-suffopheny l)-2H-tetrazolium) and NRU (neutral red uptake) assays following 24 h incubation. Airborne IC50 (50% inhibitory concentration) values were determined using dose response curves for xylene (IC50 = 5350 +/- 328 ppm, NRU; IC50 = 5750 +/- 433 ppm, NITS in skin fibroblast) and toluene (IC50 = 10 500 +/- 527 ppm, NRU; IC50 = 11200 +/- 1044 ppm, NITS in skin fibroblast). Our findings suggest that static direct exposure at the air/liquid interface is a practical and reproducible technique for toxicity testing of VOCs. Further, this technique can be used for inhalational and dermal toxicity studies of volatile chemicals in vitro as the exposure pattern in vivo is closely simulated by this method. en_US
dc.identifier.issn 1464-0325 en_US
dc.language English
dc.language.iso EN en_US
dc.rights CC BY-NC-ND 3.0 en_US
dc.rights.uri en_US
dc.source Legacy MARC en_US
dc.subject.other In vitro cytotoxicity. en_US
dc.subject.other MTS tetrazolium salt assay. en_US
dc.subject.other Neutral red uptake. en_US
dc.subject.other Volatile organic compounds (VOCs). en_US
dc.title A novel in vitro exposure technique for toxicity testing of selected volatile organic compounds en_US
dc.type Journal Article en
dcterms.accessRights metadata only access
dspace.entity.type Publication en_US
unsw.identifier.doiPublisher en_US
unsw.relation.faculty Science
unsw.relation.ispartofissue 1 en_US
unsw.relation.ispartofjournal Journal of Environmental Monitoring en_US
unsw.relation.ispartofpagefrompageto 100-105 en_US
unsw.relation.ispartofvolume 8 en_US
unsw.relation.originalPublicationAffiliation Bakand, S. en_US
unsw.relation.originalPublicationAffiliation Winder, C. en_US
unsw.relation.originalPublicationAffiliation Khalil, Christian, Safety Science, Faculty of Science, UNSW en_US
unsw.relation.originalPublicationAffiliation Hayes, A. en_US School of Risk & Safety Science *
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