Engineering

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Now showing 1 - 10 of 12
  • (2021) Chua, Stephanie
    Thesis
    Improvements in liquid lithium-ion battery electrolytes using of metal organic frameworks (MOFs) as a functional decoration on polymer membrane separators were investigated using a combination of experimental and theoretical methods. Zirconium-based MOF UiO-66 was introduced to the polymer support using the mixed matrix membrane (MMM) method. The method allowed the one-step manufacture of a robust, mechanically pliable polymer-MOF membrane composite of high MOF loading. MOF-MMMs imparted improved electrochemical behaviours such as a widened potential operating window, near-unity transference number, and increased presence of solid electrolyte interphase (SEI) components crucial to battery performance. Density functional theory (DFT) calculations were also performed to provide insights regarding electrolyte solvation in the presence of MOF. A simple dip-coating technique was utilised to modify the surface of the MOF-MMMs with polydopamine (PDA) for further improvement of the electrochemical properties. Increased transference numbers, as well as stability during rate cycling, were observed with the resulting PDA-MMM owing to the improved electrode/electrolyte interface. However, surface analyses using x-ray photoelectron spectroscopy (XPS) showed that there are reduced amounts vital SEI components compared to the original MOF-MMM support. The last section further explores the versatility of UiO-66 and tackled the preparation of gel polymer electrolytes (GPEs) decorated with UiO-66 via phase inversion technique. Using the phase inversion method, the fabricated GPE contained pores from both polymer substrate and the intrinsic pores of the 3D nanomaterial for improvement of electrochemical properties. It was demonstrated in this work that the MOF GPE is equally inert and suitable in ether or carbonate-based electrolytes. Overall, this study demonstrated the versatility of UiO-66 metal organic frameworks for use as a functional nanofiller for electrolyte membranes. With the use of inexpensive membrane fabrication methods, the composites obtained are viable for lithium-metal battery applications. Similarly, insights drawn can provide a springboard towards future study of MOF-based electrolytes.

  • (2022) Nguyen, Minh Triet
    Thesis
    Singlet fission is a photo-physical process that generates two triplet excitons from one singlet exciton and can potentially enhance efficiency in photovoltaic systems. The combination of photovoltaics and singlet fission is a novel field for solar energy conversion when there is much interest in renewable, non-destructive, and continuously available energy sources. Singlet fission can also overcome thermalization losses in photovoltaics, which happens in traditional cells when the incident photon energy is higher than the silicon bandgap energy, using a carrier multiplication mechanism. This thesis will design, construct, and characterize photovoltaic devices incorporating singlet fission materials to study singlet fission in practical application. The research focuses on materials characterization, spin dynamics, and electron transfers between acene and the semiconductor layer in Au/TiO2 ballistic cells, and the incorporation of singlet fission layers on silicon-based cell structures. In detail, a set of investigations was developed and summarized by implementing singlet fission materials into a state-of-the-art ballistic photovoltaic device and silicon-based solar cell. The studies demonstrate proof of concept and rationally explain the process. The first part of the thesis investigates thin films of pentacene, TIPS-pentacene, and tetracene via crystallinity, morphology, absorption, and thickness characterization. Additionally, Au and TiO2 layers in Schottky device structures were optimized to achieve the best performance for energy transfer from an applied dye layer (merbromin). The drop-casted dye layer influences the device performance by increasing short-circuit current and open-circuit voltage, demonstrating the ability of charge transfer between the device and the applied film. This device structure provides a test bed for studying charge and energy transfer from singlet fission films. The latter part of the thesis describes several investigations to understand singlet fission in a thin film using this architecture. Magneto-photoconductivity measurements were primarily used to observe the spin dynamics via photoconductivity under an external magnetic field. Control experiments with bare Au/TiO2 devices showed no observable magneto-photoconductivity signal. In contrast, devices with pentacene and tetracene singlet fission layers showed a strong magnetoconductivity effect caused by ballistic electron transfer from the singlet fission layer into the TiO2 n-type semiconductor through an ultra-thin gold layer inserted between the layers. A qualitatively different behavior is seen between the pentacene and tetracene, which reveals that the energy alignment plays a crucial part in the charge transfer between the singlet fission layer and the device. The last section investigates the application of pentacene and tetracene evaporated thin-films as sensitizer layers to a silicon-based solar cell. The optimized Si cell structure with the annealing treatment improved the cell's performance by increasing short-circuit current and open-circuit voltage. The deposition of pentacene and tetracene as sensitizer layers into the device showed some results but posed several challenges that need to be addressed. As the current-voltage and external quantum efficiency measurements were taken, it was observed that material interfaces need to be designed to fully achieve the singlet fission of the acene layer into the Si devices.

  • (2022) Qiao, Laicong
    Thesis
    There has been a rapid-growing market and academic enthusiasm for small wearable molecular diagnostic platforms driven by the growing demand for continuous monitoring of human health. Wearable devices need to be portable, stretchable, and ideally re-configurable to be able to work for different analytes. Such flexible physiological monitoring devices which are non-invasive or minimally-invasive represent the next frontier of biomedical diagnostics. They may make it possible to predict and prevent diseases or facilitate treatment by diagnosing diseases at the initial stages. However, there are many problems that restrict further applications of these devices. Firstly, there are a limited number of bio-materials which are highly flexible, biocompatible and have anti-fouling properties; such biomaterials are needed as substrates for wearable devices. Secondly, traditional biosensors used in wearable devices focus on the detection of physical signals (such as heartbeat) and small chemical molecules, e.g. Na+, K+. These are not sufficient to provide in depth health information which requires sensing of large molecules such as proteins, ideally in real time, which is currently challenging. This provides a motivation to develop highly sensitive wearable biosensors for the detection of large molecules in sweat. This thesis centres on the development of a bio-material based wearable device for continuous detection of crucial analytes in human sweat. To achieve this target, our first aim was to design a highly bio-compatible flexible material as a substrate for wearable devices. A tough and anti-fouling three-network hydrogel has been prepared by integrating a zwitterionic polymer network into a robust double-network hydrogel. Secondly, to fill the gap between technological development of continuous and non-invasive detection of different analytes in human sweat, a patterned sweat-based biosensor was created for the detection of key biomolecules. This sensor was produced by placing specific aptamers or enzymes on flexible working electrodes. In addition, nanotechnology methods have been applied to refine the bio-sensing interface to further increase the sensitivity of our sensors. Finally, a sample collection chip has been combined with our high sensitivity sensors to fabricate a wearable device for sweat bio-sensing purposes. Future research may involve integration of a commercially available wireless signal readout module with this wearable biosensing device. The outcomes of this work may provide new insights for the development of wearable devices for continuous measurement of a spectrum of analytes in sweat, as an important step towards point-of-care diagnostics

  • (2022) Melodia, Daniele
    Thesis
    Antibodies are increasingly useful therapeutics, and examples include the checkpoint inhibitors pembrolizumab1 and ipilimumab2 in cancer immunotherapy, and anti tau therapies in Alzheimer’s disease and dementia.3,4 However, specific applications requiring cytosolic delivery of the antibody, or transport across the blood-brain barrier pose challenges to antibody therapeutics. These issues may reduce the effectiveness of immunotherapy and restrict it to extracellular targets. Conjugating polymers to proteins and enzymes has been very effective at improving their stability and pharmacokinetics,5–8 and similar approaches have been studied for antibody conjugation.9–12 Finding an effective polymeric delivery system for antibodies can greatly improve immunotherapy. In this work three strategies were explored for the encapsulation and bioconjugation to antibodies. The first approach is the encapsulation via electrostatic interactions between the antibody and a charged block copolymer to form polyion complex (PIC) micelles. Polyphosphonium block copolymers were studied for the first time to encapsulate antibodies, and were compared to their ammonium counterpart. While this approach has the advantage of being reversible, the polymer-antibody electrostatic interactions were too weak for biological applications, and delivery by this means would require a crosslinking strategy. The second approach involves covalent attachment of polymers on the antibody’s surface via a grafting from polymerisation. An oxygen tolerant technique was employed for the screening of a large number of samples in low volumes (<100 μL). Successful grafting was demonstrated by AF4 and gel electrophoresis. Enzyme-linked immunosorbent assay (ELISA) showed retention of up to 40% binding activity relative to the native antibody with a marked improvement in stability. The third strategy introduces a novel acid sensitive linker for the reversible covalent attachment of polymers to the antibody’s surface. This was achieved by using Diels-Alder chemistry to create an activated PEG that forms an amide with a conformational lock similar to citraconic anhydride upon conjugation to the amines on the antibody. The ability of the linker to cleave at pH 5.5 is demonstrated, resulting in almost complete recovery of the original binding activity of the antibody. Overall, the reversible covalent attachment investigated here seems the most promising, and combining the high throughput method with the cleavable linker approach holds great potential for advancing in immunotherapy. References (1) Reck, M. Pembrolizumab as First-Line Therapy for Metastatic Non-Small-Cell Lung Cancer. Futur. Med. 2018, 10, 93–105. (2) Gao, J.; Ward, J. F.; Pettaway, C. A.; Shi, L. Z.; Subudhi, S. K.; Vence, L. M.; Zhao, H.; Chen, J.; Chen, H.; Efstathiou, E.; Troncoso, P.; Allison, J. P.; Logothetis, C. J.; Wistuba, I. I.; Sepulveda, M. A.; Sun, J.; Wargo, J.; Blando, J. VISTA Is an Inhibitory Immune Checkpoint That Is Increased after Ipilimumab Therapy in Patients with Prostate Cancer. Nat. Med. 2017, 23 (5), 551–555.. (3) Pedersen, J. T.; Sigurdsson, E. M. Tau Immunotherapy for Alzheimer’s Disease. Trends Mol. Med. 2015, 21 (6), 394–402. (4) Castillo-Carranza, D. L.; Sengupta, U.; Guerrero-Munoz, M. J.; Lasagna-Reeves, C. A.; Gerson, J. E.; Singh, G.; Estes, D. M.; Barrett, A. D. T.; Dineley, K. T.; Jackson, G. R.; Kayed, R. Passive Immunization with Tau Oligomer Monoclonal Antibody Reverses Tauopathy Phenotypes without Affecting Hyperphosphorylated Neurofibrillary Tangles. J. Neurosci. 2014, 34 (12), 4260–4272. (5) Abolmaali, S. S.; Tamaddon, A. M.; Salmanpour, M.; Mohammadi, S.; Dinarvand, R. Block Ionomer Micellar Nanoparticles from Double Hydrophilic Copolymers, Classifications and Promises for Delivery of Cancer Chemotherapeutics. Eur. J. Pharm. Sci. 2017, 104 (January), 393–405. (6) Kurakhmaeva, K. B.; Djindjikhashvili, I. A.; Petrov, V. E.; Balabanyan, V. U.; Voronina, T. A.; Trofimov, S. S.; Kreuter, J.; Gelperina, S.; Begley, D.; Alyautdin, R. N. Brain Targeting of Nerve Growth Factor Using Poly(Butyl Cyanoacrylate) Nanoparticles. J. Drug Target. 2009, 17 (8), 564–574. (7) Jiang, Y.; Fay, J. M.; Poon, C. D.; Vinod, N.; Zhao, Y.; Bullock, K.; Qin, S.; Manickam, D. S.; Yi, X.; Banks, W. A.; Kabanov, A. V. Nanoformulation of Brain-Derived Neurotrophic Factor with Target Receptor-Triggered-Release in the Central Nervous System. Adv. Funct. Mater. 2017, 1703982, 1–11. (8) Klyachko, N. L.; Manickam, D. S.; Brynskikh, A. M.; Uglanova, S. V.; Li, S.; Higginbotham, S. M.; Bronich, T. K.; Batrakova, E. V.; Kabanov, A. V. Cross-Linked Antioxidant Nanozymes for Improved Delivery to CNS. Nanomedicine Nanotechnology, Biol. Med. 2012, 8 (1), 119–129. (9) Bin Liu, Khushboo Singh , Shuai Gong , Mine Canakci, Barbara A. Osborne, and S. T. Protein Antibody Conjugates PACs A Plug‐and‐Play Strategy for Covalent Conjugation and Targeted Intracellular Delivery of Pristine Proteins. Angew. Chemie 2021, 133, 12923–12928. (10) Chan, L. J.; Bulitta, J. B.; Ascher, D. B.; Haynes, J. M.; Mcleod, V. M.; Porter, C. J. H.; Williams, C. C.; Kaminskas, L. M. PEGylation Does Not Signi Fi Cantly Change the Initial Intravenous or Subcutaneous Pharmacokinetics or Lymphatic Exposure of Trastuzumab in Rats but Increases Plasma Clearance after Subcutaneous Administration. Mol. Pharm. 2015, 12, 794–809. (11) Subasic, C. N.; Ardana, A.; Chan, L. J.; Huang, F.; Scoble, J. A.; Butcher, N. J.; Meagher, L.; Chiefari, J.; Kaminskas, L. M.; Williams, C. Poly ( HPMA- Co -NIPAM ) Copolymer as an Alternative to Polyethylene Glycol-Based Pharmacokinetic Modulation of Therapeutic Proteins. Int. J. Pharm. 2021, 608 (September), 121075. (12) Keita Hironaka,a,b Erika Yoshihara, Ahmed Nabil, James J. Lai, A. K. and M. E. Conjugation of Antibody with Temperature-Responsive Polymer via in Situ Click Reaction to Enable Biomarker Enrichment for Increased Diagnostic Sensitivity. Biomater. Sci. 2021, 9, 4870–4879.

  • (2022) Wulandari, Erna
    Thesis
    Chronic wounds are a major issue in public health. One of the contributing factors in the development of chronic wounds is bacterial infection, which is exacerbated by the presence of multidrug-resistant (MDR) bacteria. One approach to tackle wound infection is the use of non-antibiotic antimicrobials with rapid killing effect without inducing resistance. This thesis aims to investigate the application of antimicrobial polymers and iodine in the development of antimicrobial wound dressing platforms. Firstly, contact-active antimicrobial wound dressings were explored. An inert silk sponge was prepared as the substrate and functionalized with antimicrobial polymers on the surface via layer-by-layer assembly. Electrostatic interactions in the multilayer construct confined the antimicrobial polymers and prevented leaching. The sponge was able to suck bacteria into the porous network and kill them upon contact as evidenced by up to 4 log10 reduction against Gram-negative and Gram-positive bacteria. Additionally, the antimicrobial efficacy was found to be strongly affected by the construction of multilayer assembly. As the contact-active mechanism may reach saturation point on the surface, in the second approach, an antimicrobial platform with a release-killing mechanism was developed. Employing the ability of silk to self-assemble into a thin film, antimicrobial polymers were loaded in the silk matrix. The release of antimicrobial polymers correlated to polymer concentration, silk to polymer ratio, and film configuration. The efficacy of the films was demonstrated by 5 to 7 log10 reduction of planktonic and 3 to 7 log10 reduction of biofilm cells against Pseudomonas aeruginosa and Staphylococcus aureus, including MDR strains. Furthermore, the straightforward coating method was as effective on glass or cotton substrates. The third approach investigated the immobilization of iodine onto wound dressings for a sustained release system. The immobilization was facilitated by polyamide iodophors that were synthesized on the dressing via plasma polymerization of the gaseous amide monomers. The antimicrobial activity correlated strongly to the structure of the polyamide with the short and linear polymer recorded 4 log10 reduction against P. aeruginosa and 7 log10 reduction S. aureus, including a MDR strain. Overall, the immobilization of iodophors on wound dressings demonstrated a potential new approach in reducing bacteria proliferation in wounds.

  • (2023) Wimberger, Laura
    Thesis
    This work explores how reversible light-induced pH changes can be increased and applied to control pH-responsive systems with light. Chapter 2 investigates how substitution patterns influence the acidity of merocyanine photoacids in the dark as well as under light irradiation. The parameters which are crucial for increasing light-induced pH changes are defined and applied to synthesized merocyanine photoacids. The light-induced pH changes starting from varying initial pH values are explored and a model is developed to estimate these based on experimentally defined parameters. Transient absorption spectroscopy was used to explore the influence of the protonation state of the merocyanine form (MCH vs MC) on the photoswitching efficiency. A python model is developed to describe the pH- recovery in the dark. Chapter 3 introduces an improved merocyanine photoacid, designed by principles outlined in Chapter 2. The acidity parameters and photoswitching abilities are characterized. The pH switching capacity is investigated and extended into the basic pH range. The light-induced pH switch by the improved photoacid is applied to control the protonation state of an indicator dye. Chapter 4 applies light-induced pH changes to influence the secondary structure of pH-sensitive DNA. The transient formation of these structures is explored. The influence of the initial pH value and a DNA binder on the ratio and kinetics of the system components is investigated. Chapter 5 applies light-induced pH changes to influence the properties of different types of supramolecular polymers. The challenges of applying merocyanine photoacids to generate structural changes of supramolecular polymers by influencing the components protonation state are highlighted. Chapter 6 presents brief conclusions and a future outlook for this research field.

  • (2023) Siddique, Arslan
    Thesis
    Epidemic thunderstorm asthma (ETSA) outbreaks are triggered by airborne pollen allergens combined with thunderstorm activity. ETSA can affect anyone, as observed in the world’s largest ETSA event in Australia. Allergens from rye grass pollen affect the respiratory airways and the fundamental physicochemical causes, biochemical interactions, and the role of the thunderstorm in ETSA have been the source of much speculation. In this thesis, the physicochemical interactions of thunderstorm-derived reactive oxygen nitrogen species (RONS) and pollen-derived molecules are examined. It is hypothesised that RONS from the plasma-activated water (PAW) react with the airborne pollen allergens, exerting physicochemical changes to enhance allergenicity and subsequently causing ETSA. Simple biomimetic models are demonstrated, examining the key biointerfacial interactions and the influences of the conditions of plasma formation, pH, and temperature, employing advanced interface-sensitive techniques including QCM-D and neutron reflectometry. Firstly, cellulose-mucin interactions were analysed, mimicking the interactions between the walls of inhaled pollen (intine) and mucosa of the respiratory tract (mucin). Interaction with plasma-treated cellulose surfaces led to adsorption and conformational alterations to mucin, potentially indicating changes to the permeability of the mucosa. Secondly, the effect of PAW on the interactions between a model-allergen plant protein and lipid monolayers mimicking alveolar surfactant was studied. The protein took up RONS and PAW-treated protein showed stronger adsorption to the lipid monolayers, implying PAW-treatment enhances transport of the protein into lung tissue. Lastly, the effect of PAW on allergen penetration into epithelial bilayers was elucidated. Solid-supported model lipid bilayers were allowed to interact with model allergen and rye grass derived proteins to deduce the structural integrity of the membrane. PAW-treatment increased adsorption of the proteins to the lipid bilayers, and enabled the penetration into the membrane, corroborating the enhanced allergenicity of PAW-treated allergens. Overall, PAW was seen to enhance three relevant nonspecific biointerfacial interactions; these physicochemical studies complement extant in vitro cell studies in an effort to enable the development of effective monitoring platforms, diagnostics, and therapeutic interventions for the prevention and treatment of ETSA.

  • (2023) Zhang, Diana
    Thesis
    Early disease diagnosis can significantly improve patient survival rates as appropriate treatment strategies can be timely administered. A promising approach for disease diagnosis is to analyse chemical biomarkers present in bodily fluids as these molecules can provide insights into human metabolic and physiological processes. Changes in the identity and concentrations of such chemicals can help distinguish healthy from disease states. However, some current methods used to collect, analyse, and identify these chemicals have been challenged by limitations in sampling protocols, the resolving power of instruments, and the ability to interpret advanced data analysis methods. This thesis comprises of five concurrent efforts to enhance diagnostic accuracy by investigating various machine learning and analytical approaches. Firstly, an interpretable machine learning framework for binary disease classification is presented. Using this framework on blood plasma and skin sebum data, the diagnostic performance for Parkinson’s disease and key disease biomarkers are reported. Secondly, a protocol and recommendations for robust skin sebum analysis is described. Following a semi-longitudinal study, the various factors that can impact the collection and detection of volatile organic compounds present in skin sebum is discussed. Thirdly, the clinical utility of high-field asymmetric waveform ion mobility spectrometry (FAIMS) for disease diagnosis is reported. Based on a systematic review and meta-analysis, the diagnostic accuracy and clinical implications of using FAIMS is discussed. Fourthly, the performance of high-resolution FAIMS resulting in enhanced ion separations is reported. Using high-resolution FAIMS, the fundamentals that govern the separation of protonation protein isomers is described. Finally, the use of high-resolution FAIMS to analyse volatile organic compounds present in exhaled breath is demonstrated. Using atmospheric pressure chemical ionisation coupled with high-resolution FAIMS, untargeted breath analysis on individual breath profiles is reported. Overall, by improving analytical and machine learning methods, these findings should increase diagnostic accuracy and enable greater confidence in biomarker analysis.

  • (2023) Kaltbeitzel, Jonas
    Thesis
    Proteins and enzymes are highly versatile materials that are involved in essentially every biological process, making them valuable tools and targets in the field of medicine. This thesis explores two distinct aspects of their applications: Part I focuses on the formation of responsive nanoparticles for drug delivery, while Part II delves into the development of small molecular inhibitors and their use in a novel protease assay. Each part will start with a separate literature review, to give the reader a brief background about the topic. Their biocompatibility, non-toxicity, and ability to specifically interact with cellular receptors make proteins and enzymes promising materials in the design of nanoparticles for drug-delivery applications. In many cases, a covalent modification of the protein is required to drive the formation of nanoparticles which can inadvertently change the properties of the underlying protein. One possibility to overcome this problem is to make the covalent modification reversible by the introduction of responsive linker molecules, which additionally allows targeting. Therefore, Part I of this thesis will explore nanoparticles that degrade in response to specific environmental cues, such as reducing agents, UV-light, or hypoxia. The first chapter is a comprehensive review of the literature on different protein-nanoparticle and the use of responsive linker systems in drug delivery applications. Chapter 2 of the thesis will present the synthesis of PEGylated enzyme nanoparticles designed for delivering catalytically active enzymes into cells. The results obtained will demonstrate the triggered disassembly of the nanoparticles and the subsequent release of catalytically active enzymes, leading to cellular toxicity. Moving on to Chapters 3 and 4, reductive-responsive nanoparticles composed of bovine serum albumin (BSA) and a hypoxia-responsive polymer will be featured as an intracellular drug delivery vehicle for nucleic acids. In Part II of the thesis, the focus is shifted to the design of small molecule inhibitors for acetylcholinesterase (AChE) and their use in the development of a novel protease assay. AChE has important implications in the treatment of Alzheimer's and other diseases. After a short literature review in Chapter 5 discussing the enzyme and past development of its inhibitors, Chapter 6 shows the journey in the design of potent, primary amine-containing inhibitors of AChE based on several known scaffolds. The increased polarity of the molecules hinders their ability to cross the blood-brain barrier, suggesting a potential application in the treatment of functional dyspepsia. Lastly, Chapter 7 deals with the development of a new proteases assay based on the inhibitors synthesized in the previous chapter. Proteases play a crucial role in many biological processes and are thus important medical markers for various diseases. The effect of the potency of the inhibitors after covalent modification with short peptides was evaluated and a mathematical model developed to predict the sensitivity of the assay.

  • (2023) Islam, Md Shariful
    Thesis
    Tissue engineering aims to create functional tissues by cultivating cells in a laboratory setting. A primary area of focus to achieve that objective is the development of scaffolds capable of providing a suitable environment for cellular adhesion, growth, and the execution of fundamental cellular functions to establish tissue-scale properties. However, scaffold systems in the laboratory do not benefit from the dynamic forces that are exerted on tissues in an organism. In pursuit of this aim, the overall objective of this thesis was to develop a tissue engineering scaffold system mimicking the natural tissue-like environment, with in-built capabilities for external control of dynamic mechanical properties to modulate cell differentiation. We first developed a magnetic nanoparticle-loaded hydrogel system, where the modulus of the hydrogels can be reversibly altered by applying a magnetic field. To demonstrate versatility, we have used two popular hydrogel systems broadly used in tissue engineering: poly (ethylene glycol dimethacrylate) and gelatine methacryloyl. We analysed the effects of the field-induced change in stiffness on cell behaviour upon the attenuation of a magnetic field. Our studies demonstrate that adipose-derived stem cells (ADSC) and embryonic muscle cells (C2C12 cell line) can perceive these stiffness changes and differentiate towards myofibroblast and myoblast, respectively. We then developed a composite hydrogel system to segregate the magnetic particles within gelatin fibres, which simultaneously provides nanotopography to the adherent cells. We used electrospinning to synthesize magnetic gelatin nanofibers containing 5 wt/v% iron oxide nanoparticles. This concentration was selected to ensure maintenance of fiber morphology while simultaneously ensuring magnetic response. To stabilize the nanofiber structure, we used a crosslinking method involving citric acid and high temperature to stabilize the gelatin via amide bonds between strands. Introducing a magnetic nanofiber mat at the interface of the hydrogel system provides remote actuation of the nanotopography through an external magnetic field. We found that the nanotopography alone directed adipogenesis, while mechanical actuation of the interface drove osteogenesis in adherent ADSCs. The adhesion characteristics suggest that the field influences the nanofiber structure, greatly enhancing focal adhesion. The field induced actuation was also found to stimulate the formation of aligned multinucleated myotubes and markers associated with maturation in adherent C2C12. Finally, we integrated the magnetic nanofiber into hydrogels as a modular system that closely resembles the fibrous network in the natural extracellular matrix. These hydrogels can be reversibly stiffened in response to external magnetic fields within cell-laden 3D constructs. Including a small fraction of short nanofibers (<3%) can significantly influence ADSC and C2C12 differentiation. As before, nanotopography was beneficial to adipogenesis while stiffening promoted enhanced osteogenesis and myogenesis. Together, this body of work provides a modular platform with broad versatility in format to study the effects of nano-topography and dynamic mechanics in cell systems. Moreover, these hydrogels and the magnetic components are cytocompatible with scope for inclusion in tissue bioreactors as a means for dynamic stimulation of cell differentiation for tissue engineering.