The biological effects of micro- and nanoparticles in sunscreens products on human skin

dc.contributor.other Pattanamahakul, Preprame en_US
dc.contributor.other Bakand, Shahnaz en_US
dc.contributor.other Dechsakulthorn, Fin en_US 2021-11-26T10:33:35Z 2021-11-26T10:33:35Z 2011 en_US
dc.description.abstract The histopathological responses of human skin were studied in vitro following topical application of sunscreen products containing selected micro- and nanoparticles particles. Adult human skin (Caucasian, age 35-55) donated following elective radical abdominoplasty was used. A Franz cell diffusion apparatus (PermeGear Inc; Hellertown, PA, USA) was used to assess histopathological and morphological changes to the human skin. en_US
dc.language English
dc.language.iso EN en_US
dc.rights CC-BY-NC-ND en_US
dc.rights.uri en_US
dc.subject.other Nanotoxicology en_US
dc.subject.other Sunscreen en_US
dc.subject.other Nanoparticles en_US
dc.title The biological effects of micro- and nanoparticles in sunscreens products on human skin en_US
dc.type Dataset en_US
dcterms.accessRights metadata only access
dcterms.rightsHolder University of New South Wales en_US
dspace.entity.type Dataset en_US
unsw.contributor.leadChiefInvestigator Hayes, Amanda en_US
unsw.contributor.researchDataCreator Hayes, Amanda en_US
unsw.coverage.temporalFrom 2006 en_US
unsw.coverage.temporalTo 2010 en_US
unsw.description.storageplace Faculty of Science, UNSW Australia, Sydney NSW 2052 en_US
unsw.isDatasetRelatedToDataset In vitro toxicity data of the selected micro and nano size sunscreen particles with human cells
unsw.isDatasetRelatingToDataset The characterisation data of selected ZnO and TiO2 particles
unsw.relation.OriginalPublicationAffiliation Pattanamahakul, Preprame, , External, en_US
unsw.relation.OriginalPublicationAffiliation Bakand, Shahnaz, School of Chemistry, Faculty of Science, en_US
unsw.relation.OriginalPublicationAffiliation Hayes, Amanda, School of Chemistry, Faculty of Science, en_US
unsw.relation.OriginalPublicationAffiliation Dechsakulthorn, Fin, , This record is inactive, as the person is not currently at UNSW., en_US
unsw.relation.faculty Other UNSW
unsw.relation.faculty Science
unsw.relation.projectDesc The rapid expansion of nanotechnology has led to a variety of nanoparticles and nanomaterial products with novel physicochemical characteristics. Sunscreen application benefits largely from nanoscales of zinc oxide (ZnO) and titanium dioxide (TiO2) but toxicological profiles of these nanomaterial products are still poorly characterised. This research explores the potential of in vitro methods for toxicity assessment of ZnO and TiO2 sunscreen products. A tiered approach for toxicity testing of sunscreen particles was developed using in vitro assays and skin penetration models. Cytotoxicity was assessed using human skin fibroblasts and A549 lung cells and a range of bioassays (MTS, NRU, ATP and LDH). Physicochemical characteristics of test particles were investigated using analytical techniques. The skin penetration of customised and commercial sunscreens was investigated on freshly excised human abdominal skin using a Franz cell diffusion apparatus followed by three staining techniques (Hematoxylin and Eosin, Gomori trichrome and van Gieson). The results demonstrated that ZnO particles were more toxic than TiO2 particles with regards to altering mitochondrial activities, damaging cell membranes and causing cell death. ZnO nanoparticles (IC50 = 6.64 ± 0.37 ppm) were found to be more toxic than ZnO microparticles (IC50 = 24.66 ± 2.56 ppm). Zn ions were not significantly responsible for cell viability reduction indicating that cytotoxicity was mainly due to particulates rather than released ions. The ATP assay was the most sensitive bioassay selected. SEM/TEM and other techniques revealed that the smaller hydrodynamic size of ZnO nanoparticles could potentially relate to the higher toxicity when compared to microparticle diameters. Although both sunscreen particles played an important role in UVB protection, photomicrographs of human skin suggested that ZnO and TiO2 nanoparticles penetrated through the epidermis following topical application. The significant keratinocyte solubilisation observed was also an indication of adverse effects. Therefore, the use of nanomaterials in sunscreens needs to be regulated and toxicity of nano-products should be evaluated as a very early stage of product development using appropriate test methods. In vitro methods developed in this thesis coupled with the Franz cell diffusion technique could potentially be implemented for toxicity screening strategies of nanoparticles with cosmetic applications. en_US
unsw.relation.projectEndDate 2011-12-31 en_US
unsw.relation.projectTitle Development of in vitro toxicity test method for safety evaluation of nanoparticles in sunscreen products en_US School of Chemistry
unsw.subject.fieldofresearchcode 100713 Nanotoxicology, Health and Safety en_US
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