This DATSETNAMEreadme.txt file was generated on YYYY-MM-DD by NAME
<help text is included in angle brackets, and can be deleted before saving>


GENERAL INFORMATION

1. Title of Dataset: Inter- and Intra-observer agreement between embryologists for cytoplasmic string assessment in day 5/6 human blastocysts 

2. Author Information
	A. Principal Investigator Contact Information
		Name: Jessica Eastick
		Institution: IVF Australia
		Address: PO Box 417 New Lambton, New South Wales, 2305 Australia
		Email: Jessica.eastick@hunterivf.com.au

	B. Associate or Co-investigator Contact Information
		Name: Christos Venetis
		Institution: IVF Australia
		Address: Level 3/15 Bowden St, Alexandria NSW 2015
		Email: christos.venetis@ivf.com.au

	C. Alternate Contact Information
		Name: Simon Cooke 
		Institution: IVF Australia
		Address: Level 2/176 Pacific Hwy, Greenwich NSW 2065
		Email: simon.cooke@ivf.com.au

3. Date of data collection (single date, range, approximate date) <suggested format YYYY-MM-DD>: 2019-11-01 to 2020-09-01

4. Geographic location of data collection <latitude, longiute, or city/region, State, Country, as appropriate>: Newcastle, NSW Australia

5. Information about funding sources that supported the collection of the data: none to declare


SHARING/ACCESS INFORMATION

1. Licenses/restrictions placed on the data: CC BY

2. Links to publications that cite or use the data: Nil

3. Links to other publicly accessible locations of the data: Nil

4. Links/relationships to ancillary data sets: NO

5. Was data derived from another source? no 
	A. If yes, list source(s): 

6. Recommended citation for this dataset: 


DATA & FILE OVERVIEW

1. File List: 
<list all files (or folders, as appropriate for dataset organization) contained in the dataset, with a brief description> 

2. Relationship between files, if important: nil

3. Additional related data collected that was not included in the current data package:  NO

4. Are there multiple versions of the dataset? no
	A. If yes, name of file(s) that was updated: 
		i. Why was the file updated? 
		ii. When was the file updated? 


METHODOLOGICAL INFORMATION

1. Description of methods used for collection/generation of data: 
<Include links or references to publications or other documentation containing experimental design or protocols used in data collection> This was a prospective study involving five embryologists working in one IVF clinic between November 2019 and September 2020. Inter-observer agreement was calculated using assessments performed by five embryologists on 104 day 5/6 blastocysts regarding the presence, the number and location of CS as well as CS vesicle activity using timelapse videos produced from the Embryoscope+. Intra-observer agreement was calculated when the same embryologists repeated the same observations after a month’s break. Inter-and intra-observer agreement was assessed using Fleiss’ Kappa coefficient and the Intra-Class Correlation Coefficient (ICC).

2. Methods for processing the data: 
<describe how the submitted data were generated from the raw or collected data> survey data collected from embryologists in excel format

3. Instrument- or software-specific information needed to interpret the data: 
<include full name and version of software, and any necessary packages or libraries needed to run scripts> Microsoft Excel

4. Standards and calibration information, if appropriate: NIL

5. Environmental/experimental conditions: NIL

6. Describe any quality-assurance procedures performed on the data: NIL

7. People involved with sample collection, processing, analysis and/or submission: Jessica Eastick, Christos Venetis, Simon Cooke, Michael Chapman


DATA-SPECIFIC INFORMATION FOR: [FILENAME]
<repeat this section for each dataset, folder or file, as appropriate>

1. Number of variables: 15

2. Number of cases/rows: 104

3. Variable List: 
<list variable name(s), description(s), unit(s)and value labels as appropriate for each>
Quality - blastocyst quality category
Stage - blastocyst grade
ICM- ICM grade of blastocyst
TE - TE grade of blastocyst
CS present - category - y/n
Time start - hours
time end - hours
start stage of development - blastocyst stage
end stage of development - blastocyst stage
duration - hours
Total number CS seen - numerical value
vesicles present - categorical y/n
Max no vesicles seen - numerical value
Direction of vesicle - category
Region of blast CS located - category


4. Missing data codes: 
<list code/symbol and definition> NIL

5. Specialized formats or other abbreviations used: CS; cytoplasmic strings, ICM inner cell mass, TE trophectoderm cells